Macrophage / Histiocytoma Marker 抗体
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- 抗原 See all Macrophage / Histiocytoma Marker products
- Macrophage / Histiocytoma Marker
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Macrophage / Histiocytoma Marker antibody is un-conjugated
- 应用范围
- Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- In Western blotting, it detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
- 无交叉反应
- 小鼠, 猪, 大鼠
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Membrane preparation from human hepatocytes
- 克隆位点
- D11
- 亚型
- IgG1 kappa
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anti-Macrophage / Histiocytoma Marker antibody
适用: 人 IHC, StM 宿主: 小鼠 Monoclonal D11 unconjugated
anti-Macrophage / Histiocytoma Marker antibody适用: 人 IHC, StM 宿主: 小鼠 Monoclonal LN-5 unconjugated
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- 应用备注
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Positive Control: Liver or Histiocytoma.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM PBS without BSA and without Azide.
- 储存液
- Azide free
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- 有效期
- 24 months
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- 抗原
- Macrophage / Histiocytoma Marker
- 别名
- Macrophage & Histiocytoma Marker (Macrophage / Histiocytoma Marker 产品)
- 分子量
- 125kDa -135kDa
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