LAG3 抗体
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- 抗原 See all LAG3 抗体
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LAG3 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Coating (Coat)
- 特异性
- LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human LAG3 protein
- 克隆位点
- LAG3-3261
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product LAG3 Primary Antibody
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- 应用备注
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Known_Application: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Optimal dilution for a specific application should be determined.
Positive_Control: Human tonsil or Hodgkin's lymphoma (IHC-P).
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- 别名
- LAG3 (LAG3 产品)
- 别名
- CD223 antibody, LAG-3 antibody, Ly66 antibody, lymphocyte activating 3 antibody, lymphocyte-activation gene 3 antibody, LAG3 antibody, Lag3 antibody
- 背景
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CD223, FDC protein, LAG-3,Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)
Cellular localisation: Membrane. - 分子量
- 70kDa
- 基因ID
- 3902, 409523
- UniProt
- P18627
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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