Keratin 77 抗体
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- 抗原 See all Keratin 77 (KRT77) 抗体
- Keratin 77 (KRT77)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 77 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
- 交叉反应 （详细）
- Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken. Turtle.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Human epidermal keratin
- 克隆位点
- AE-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KRT77 Primary Antibody
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anti-Keratin 77 (KRT77) antibody
KRT77 适用: 人, 大鼠, 小鼠, 兔, Cow, 犬, 小鸡, 猴, Turtle IHC, WB, IF, FACS, StM 宿主: 小鼠 Monoclonal AE-1 unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTL-1577R unconjugated Recombinant Antibody
anti-Keratin 77 (KRT77) (Internal Region) antibodyKRT77 适用: 人, 大鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Keratin 77 (KRT77) (AA 1-578) antibodyKRT77 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Skin or squamous cell carcinoma (IHC).
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Keratin 77 (KRT77)
- 别名
- KRT77 (KRT77 产品)
- 背景
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K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)
Cellular localisation: Cytoplasmic - 分子量
- 40-56.5kDa
- 基因ID
- 374454, 334989
- UniProt
- Q7Z794
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