Cytokeratin 2 抗体
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- 抗原 See all Cytokeratin 2 (KRT76) 抗体
- Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytokeratin 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction
- 克隆位点
- DE-SQ
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KRT76 Primary Antibody
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC12 cells. Tonsil, Skin, Prostate, Esophagus, Ectocervix or Squamous carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
- 别名
- KRT76 (KRT76 产品)
- 别名
- HUMCYT2A antibody, KRT2B antibody, KRT2P antibody, 2310001L23Rik antibody, keratin 76 antibody, KRT76 antibody, Krt76 antibody
- 背景
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KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
Cellular localisation: Cytoplasmic - 分子量
- 50-67kDa
- 基因ID
- 51350, 654392
- UniProt
- Q01546
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