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Keratin 5/8 抗体

KRT5/8 适用: 人 WB, FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal C-50 unconjugated
产品编号 ABIN6937713
发货至: 中国
  • 抗原 See all Keratin 5/8 (KRT5/8) products
    Keratin 5/8 (KRT5/8)
    适用
    • 22
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    • 13
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    宿主
    • 22
    小鼠
    克隆类型
    • 22
    单克隆
    标记
    • 9
    • 4
    • 3
    • 1
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    • 1
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    This Keratin 5/8 antibody is un-conjugated
    应用范围
    • 16
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    • 8
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    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
    无交叉反应
    小鸡, 非洲爪蟾
    交叉反应 (详细)
    Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.
    免疫原
    Cytoskeletal preparation from HeLa cells
    克隆位点
    C-50
    亚型
    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

    Positive_Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    储存液
    Azide free
    储存条件
    4 °C
    储存方法
    Store at 2 to 8°C. Antibody is stable for 24 months.
    有效期
    24 months
  • 抗原
    Keratin 5/8 (KRT5/8)
    别名
    KRT5 & KRT8 (KRT5/8 产品)
    背景
    CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 5/8
    Cellular localisation: Cytoplasmic
    分子量
    58kDa (CK5), 52.5kDa (CK8)
    基因ID
    3852, 3856, 433845
    UniProt
    P13647, P05787
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