Keratin 5/8 抗体
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- 抗原 See all Keratin 5/8 (KRT5/8) products
- Keratin 5/8 (KRT5/8)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 5/8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
- 无交叉反应
- 小鸡, 非洲爪蟾
- 交叉反应 (详细)
- Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.
- 免疫原
- Cytoskeletal preparation from HeLa cells
- 克隆位点
- C-50
- 亚型
- IgG1 kappa
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store at 2 to 8°C. Antibody is stable for 24 months.
- 有效期
- 24 months
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- 抗原
- Keratin 5/8 (KRT5/8)
- 别名
- KRT5 & KRT8 (KRT5/8 产品)
- 背景
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CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 5/8
Cellular localisation: Cytoplasmic - 分子量
- 58kDa (CK5), 52.5kDa (CK8)
- 基因ID
- 3852, 3856, 433845
- UniProt
- P13647, P05787
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