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KRT 抗体

KRT126P 适用: 人 FACS, IHC, IF, IHC (f) 宿主: 小鼠 Monoclonal PCK-3150 unconjugated
产品编号 ABIN6937638
发货至: 中国
  • 抗原 See all KRT (KRT126P) products
    KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
    适用
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    宿主
    • 4
    • 3
    小鼠
    克隆类型
    • 5
    • 2
    单克隆
    标记
    • 7
    This KRT antibody is un-conjugated
    应用范围
    • 4
    • 4
    • 3
    • 3
    • 3
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    • 2
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    Anti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
    交叉反应 (详细)
    Human. Dog. Cat.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
    克隆位点
    PCK-3150
    亚型
    IgG2a kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Skin, adeno- or squamous carcinomas (IHC).

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
    别名
    KRT (KRT126P 产品)
    别名
    KRT antibody, keratin 126 pseudogene antibody, KRT126P antibody
    背景
    K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, pan (Epithelial Marker)
    Cellular localisation: Cytoplasmic
    分子量
    40-67kDa (Multiple)
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