IGHG 抗体
-
- 抗原 See all IGHG products
- IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This IGHG antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Purified human Ig Gamma Chain
- 克隆位点
- IG217
- 亚型
- IgG1 kappa
-
-
- 应用备注
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin/paraffin) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
-
- 抗原
- IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
- 别名
- IGHG (IGHG 产品)
- 别名
- AU044919 antibody, IGHG2A antibody, Immunoglobulin heavy chain (gamma polypeptide) antibody, Ighg antibody
- 背景
-
G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4,IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker)
Cellular localisation: Cytoplasm, Cell Surface and Secreted - 分子量
- 75kDa
- 基因ID
- 3500, 3501, 3502
- UniProt
- P01857, P01859, P01860, P01861
-