IGHG 抗体
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- 抗原 See all IGHG products
- IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IGHG antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Gamma heavy chain(IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Purified human IgG
- 克隆位点
- IG217-IG266
- 亚型
- IgG2a kappa
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- 应用备注
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Known_Application: Flow Cytometry (1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
- 别名
- IGHG (IGHG 产品)
- 别名
- AU044919 antibody, IGHG2A antibody, Immunoglobulin heavy chain (gamma polypeptide) antibody, Ighg antibody
- 背景
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G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4,Immunoglobulin Gamma (IgG) Heavy Chain
Cellular localisation: Cytoplasm, Cell Surface and Secreted - 分子量
- ~75kDa
- 基因ID
- 3500, 3501, 3502
- UniProt
- P01857, P01859, P01860, P01861
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