FBXO5 抗体 (AA 1-250)
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- 抗原 See all FBXO5 抗体
- FBXO5 (F-Box Protein 5 (FBXO5))
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抗原表位
- AA 1-250
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This FBXO5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
- 克隆位点
- EMI1-1176
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product FBXO5 Primary Antibody
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anti-F-Box Protein 5 (FBXO5) (AA 1-250) antibody
FBXO5 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal EMI1-1176 unconjugated
anti-F-Box Protein 5 (FBXO5) (C-Term) antibodyFBXO5 适用: 人, 犬, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5H7 unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 326-375) antibodyFBXO5 适用: 人, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (C-Term) antibodyFBXO5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 1-250) antibodyFBXO5 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal EMI1-1176 unconjugated
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- 应用备注
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Known_Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- FBXO5 (F-Box Protein 5 (FBXO5))
- 别名
- FBXO5 (FBXO5 产品)
- 别名
- EMI1 antibody, FBX5 antibody, Fbxo31 antibody, 2510044I10Rik antibody, C85305 antibody, Emi1 antibody, emi1 antibody, fc65h02 antibody, wu:fc65h02 antibody, wu:fe06e07 antibody, wu:fz79f03 antibody, zgc:136397 antibody, zgc:158541 antibody, fbx5 antibody, Fbxo5 antibody, ACYPI007854 antibody, fbxo5 antibody, F-box protein 5 antibody, F-box only protein 5 antibody, F-box protein 5 L homeolog antibody, F-box protein 5 S homeolog antibody, FBXO5 antibody, Fbxo5 antibody, fbxo5 antibody, fbxo5.L antibody, fbxo5.S antibody
- 背景
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Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31,EMI1 (Early Mitotic Inhibitor-1)
Cellular localisation: Nuclear - 分子量
- 56kDa
- 基因ID
- 26271, 520506
- UniProt
- Q9UKT4
- 途径
- Mitotic G1-G1/S Phases
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