Keratin Basic 抗体
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- 抗原 See all Keratin Basic 抗体
- Keratin Basic
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适用
- 人, 大鼠, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Solubilized keratin extract from human stratum corneum
- 克隆位点
- SPM591
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product Primary Antibody
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anti-Keratin Basic antibody
适用: 人, 大鼠 WB, IF, FACS, IHC (p), ELISA 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal SPM591 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠, 兔, Cow, 小鸡, Non-Human Primate WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal AE3 unconjugated
anti-Keratin Basic antibody适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Keratin Basic antibody适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠, 兔, Cow, 小鸡, 猴, 犬 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal AE3 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠, 兔, Cow, 小鸡, 猴, 犬 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal AE3 unconjugated
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- 应用备注
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Positive Control: PC12 cells, Skin, Prostate carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM PBS without BSA and without Azide.
- 储存液
- Azide free
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- 有效期
- 24 months
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- 抗原
- Keratin Basic
- 别名
- Cytokeratin, Basic (Type II or HMW) (Keratin Basic 产品)
- 分子量
- 67kDa (CK1), 58kDa (CK5), 56.5kDa (CK10), 50kDa (CK14)
- 基因ID
- 3848, 3852, 3858
- UniProt
- P04264, P13647, P13645, P02533
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