Cytokeratin 8/18 抗体
-
- 抗原 See all Cytokeratin 8/18 products
- Cytokeratin 8/18
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Cytokeratin 8/18 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Cytoskeletal preparation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1)
- 克隆位点
- B22-1-B23-1
- 亚型
- IgG1 kappa
-
anti-Cytokeratin 8/18 antibody
适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal B22-1-B23-1 unconjugated
anti-Cytokeratin 8/18 antibody适用: 人 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal KRT8-803-KRT18-835 unconjugated
anti-Cytokeratin 8/18 antibody适用: 人 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal KRT8-803-KRT18-835 unconjugated
-
- 应用备注
-
Positive Control: MCF-7, HeLa, PMC-42 or A431 cells. Skin, colon, lung or breast carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 缓冲液
- 10 mM PBS without BSA and without Azide.
- 储存液
- Azide free
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- 有效期
- 24 months
-
- 抗原
- Cytokeratin 8/18
- Abstract
- Cytokeratin 8/18 产品
- 分子量
- 52.5kDa (CK8), 45kDa (CK18)
- 基因ID
- 3856, 3875
- UniProt
- P05787, P05783
-
