toxA 抗体
This anti-toxA antibody is a 小鼠 单克隆 antibody detecting toxA in ELISA, IF 和 Coat. Suitable for Clostridium difficile.
Quick Overview for toxA 抗体 (ABIN6937000)
抗原
toxA
(Clostridium Difficile Toxin A (toxA))
适用
Clostridium difficile
宿主
小鼠
克隆类型
单克隆
标记
This toxA antibody is un-conjugated
应用范围
ELISA, Immunofluorescence (IF), Coating (Coat)
克隆位点
253-422
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特异性
- This MAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
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纯化方法
- Purified by Protein A/G
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免疫原
- C. difficile Toxin A
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亚型
- IgG1 kappa
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应用备注
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Positive Control: Clostridium difficile extract or infected cells. Tissue.
Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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缓冲液
- 10 mM PBS without BSA and without Azide.
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储存液
- Azide free
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
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有效期
- 24 months
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- toxA (Clostridium Difficile Toxin A (toxA))
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别名
- Clostridium difficile (Toxin A)
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分子量
- 308kDa (Toxin A)
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UniProt
- P16154
抗原
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