toxA 抗体
toxA
适用: Clostridium difficile
ELISA, IF, Coat
宿主: 小鼠
Monoclonal
253-422
unconjugated
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- 抗原 See all toxA products
- toxA (Clostridium Difficile A Toxin (toxA))
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适用
- Clostridium difficile
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This toxA antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (IF), Coating (Coat)
- 特异性
- This MAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- C. difficile Toxin A
- 克隆位点
- 253-422
- 亚型
- IgG1 kappa
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- 应用备注
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Positive Control: Clostridium difficile extract or infected cells. Tissue.
Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM PBS without BSA and without Azide.
- 储存液
- Azide free
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- 有效期
- 24 months
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