CEACAM1/5 抗体
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- 抗原 See all CEACAM1/5 products
- CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CEACAM1/5 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)
- 特异性
- This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
- 交叉反应 （详细）
- Human and Monkey.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Purified human CEA protein
- 克隆位点
- C66-195
- 亚型
- IgG1 kappa
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anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5) (N-Term) antibody
CEACAM1/5 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5) antibodyCEACAM1/5 适用: 人 FACS, WB, ELISA, cELISA, IHC (fro) 宿主: 小鼠 Monoclonal 4-3-17 unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: MCF7 or 293T cells. Human colon carcinoma
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))
- 别名
- CEACAM1 & CEACAM5 (CEACAM1/5 产品)
- 别名
- CD66e antibody, CEA antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, CEACAM5 antibody
- 背景
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Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1),Carcinoembryonic Antigen (CEA) / CD66
Cellular localisation: Cytoplasmic and luminal surface - 分子量
- 80-200kDa
- 基因ID
- 1048, 709196
- UniProt
- P06731
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