CD53 抗体
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- 抗原 See all CD53 抗体
- CD53
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD53 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunomicroscopy (IM)
- 特异性
- Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI, Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).
- 无交叉反应
- Baboon, 马
- 交叉反应 （详细）
- Human. Does not react with Baboon or Horse.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Stimulated human leukocytes
- 克隆位点
- 161-2
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CD53 Primary Antibody
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anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7A8D3 unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 1H2C7 unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 PE
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-53 unconjugated
anti-CD53 (CD53) (AA 100-180) antibodyCD53 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IF, IP, IHC 宿主: 兔 Chimeric HD77 unconjugated Recombinant Antibody
anti-CD53 (CD53) (Internal Region) antibodyCD53 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibody (FITC)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 FITC
anti-CD53 (CD53) (AA 65-160) antibodyCD53 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal 4AC53-2 PE
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- 应用备注
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Known_Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- CD53
- 别名
- CD53 (CD53 产品)
- 别名
- AI323659 antibody, Ox-44 antibody, Tspan25 antibody, MOX44 antibody, TSPAN25 antibody, OX44 antibody, CD53 antigen antibody, CD53 molecule antibody, Cd53 molecule antibody, Cd53 antibody, CD53 antibody
- 背景
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CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25,CD53 (TSPAN25)
Cellular localisation: Cell Surface - 分子量
- 33-55kDa
- 基因ID
- 963, 443057
- UniProt
- P19397
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