GLG1 抗体 (CF®647)
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- 抗原 See all GLG1 抗体
- GLG1 (Golgi Glycoprotein 1 (GLG1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GLG1 antibody is conjugated to CF®647
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)
- 特异性
- This MAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. Highest levels are expressed in pancreas, skeletal muscle, placenta, heart, testis and ovary. It is also found in the kidney, liver, lung and brain. It is expressed in both adult and fetal tissues. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
- 无交叉反应
- 小鼠, 大鼠
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Golgi fraction from human liver cells
- 克隆位点
- GLG1-970
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product GLG1 Primary Antibody
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- 应用备注
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Positive Control: HepG2, A431 or HeLa cells. Tonsil or lymph node.
Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 100 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA and 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- GLG1 (Golgi Glycoprotein 1 (GLG1))
- 别名
- GLG1 (GLG1 产品)
- 别名
- AI593353 antibody, AW537898 antibody, CFR antibody, CFR-1 antibody, ESL-1 antibody, MG-160 antibody, MG160 antibody, Selel antibody, ESL1 antibody, LTCP-1 antibody, golgi glycoprotein 1 antibody, Golgi apparatus protein 1 antibody, golgi apparatus protein 1 antibody, GLG1 antibody, LOC100175586 antibody, Glg1 antibody
- 分子量
- 134kDa
- 基因ID
- 2734
- UniProt
- Q92896
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