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TLR3 抗体 (AA 801-905)

Name: TLR3 抗体 (AA 801-905)
SKU: ABIN686617
Availability: OutOfStock

TLR3 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR3 Polyclonal Antibody, Unconjugated (ABIN686617) at 1:200 followed by conjugation to the secondary antibody and DAB staining (TLR3 抗体 (AA 801-905))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (TLR3 抗体 (AA 801-905))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (TLR3 抗体 (AA 801-905))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat lung tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (TLR3 抗体 (AA 801-905))

IHC (p)

4 images

(4 references)

产品编号 ABIN686617

发货至: 中国

Datasheet as PDF

抗原 See all TLR3 抗体

TLR3 (Toll-Like Receptor 3 (TLR3))
抗原表位
15

10

8

8

5

4

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 801-905
适用
95

61

29

6

2

1

1

1
人, 小鼠, 大鼠
宿主
80

31

3

2
兔
Compatible Secondaries
克隆类型
83

33
多克隆
标记
48

18

15

8

5

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TLR3 antibody is un-conjugated
应用范围
77

40

34

33

21

14

13

12

12

12

7

4

4

3

2
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse TLR3

亚型

IgG

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anti-Toll-Like Receptor 3 (TLR3) (AA 464-647) antibody
TLR3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 3 (TLR3) (AA 24-217) antibody
TLR3 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6F3G4 unconjugated

anti-Toll-Like Receptor 3 (TLR3) (C-Term) antibody
TLR3 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 3 (TLR3) (AA 135-297) antibody
TLR3 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 3 (TLR3) (AA 55-70) antibody
TLR3 适用: 人, 猴, 山羊, 绵羊 WB, IHC, FACS, IP, IHC (p) 宿主: 小鼠 Monoclonal 40C1285-6 unconjugated

anti-Toll-Like Receptor 3 (TLR3) (C-Term) antibody
TLR3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 3 (TLR3) (AA 750-800), (pTyr759) antibody
TLR3 适用: 人 WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 3 (TLR3) (AA 135-150) antibody
TLR3 适用: 小鼠 WB, IHC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 3 (TLR3) (AA 727-905) antibody
TLR3 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhao, Zhang, Wang, Jin, Zhou, Chen, Su: "Activation of Toll-like receptor 3 promotes pathological corneal neovascularization by enhancement of SDF-1-mediated endothelial progenitor cell recruitment." in: Experimental eye research, Vol. 178, pp. 177-185, (2019) (PubMed).

Zhu, Xu, Jiang, Wang, Geng, Wu, Hussain, Gao, Han, Li, Lan, Ning, Zhang, Holmdahl, Meng, Lu: "Pristane induces autophagy in macrophages, promoting a STAT1-IRF1-TLR3 pathway and arthritis." in: Clinical immunology (Orlando, Fla.), Vol. 175, pp. 56-68, (2016) (PubMed).

Wang, Lei, Zhang, Zhao, Fang, Lai, Han, Xiao, Wang: "Xenobiotic Pregnane X Receptor (PXR) Regulates Innate Immunity via Activation of NLRP3 Inflammasome in Vascular Endothelial Cells." in: The Journal of biological chemistry, Vol. 289, Issue 43, pp. 30075-81, (2014) (PubMed).

Zhu, Meng, Jiang, Xu, Wang, Han, Lu: "Overexpression of toll-like receptor 3 in spleen is associated with experimental arthritis in rats." in: Scandinavian journal of immunology, Vol. 76, Issue 3, pp. 263-70, (2012) (PubMed).
抗原

TLR3 (Toll-Like Receptor 3 (TLR3))

别名

TLR3 (TLR3 产品)

别名

CD283 antibody, IIAE2 antibody, AI957183 antibody, zgc:123006 antibody, toll like receptor 3 antibody, toll-like receptor 3 antibody, TLR3 antibody, Tlr3 antibody, tlr3 antibody

背景

Synonyms: AI957183, Toll-like receptor 3, CD283, Tlr3
Background: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response (By similarity).

基因ID

142980

UniProt

Q99MB1

途径

TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades