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Iba1 抗体 (AA 51-147)

Name: Iba1 抗体 (AA 51-147)
SKU: ABIN685477
Availability: OutOfStock
Rating: 5 (1 reviews)

IBA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477) (Iba1 抗体 (AA 51-147))

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (ABIN685477) followed by conjugation to the secondary antibody and DAB staining (Iba1 抗体 (AA 51-147))

Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody at 1:100 dilution. (Iba1 抗体 (AA 51-147))

IHC

11 images

(7 references)

(1 validation)

产品编号 ABIN685477

发货至: 中国

Datasheet as PDF

抗原 See all Iba1 (IBA1) 抗体

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
抗原表位
16

15

15

9

8

8

7

7

3

1

1

1

1

1

1

1
AA 51-147
适用
94

57

52

1

1
人, 大鼠, 小鼠
宿主
82

32

5
兔
Compatible Secondaries
克隆类型
86

33
多克隆
标记
49

17

13

6

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
This Iba1 antibody is un-conjugated
应用范围
71

44

38

21

16

13

13

11

11

6

4

4

3

3

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human AIF1

亚型

IgG

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IBA1 适用: 人 WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 适用: 人 WB, IHC, IP, ICC, IF, IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (Internal Region) antibody
Verified IBA1 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody
Verified IBA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 35-102) antibody
IBA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-146) antibody
IBA1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AIF1-1909 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A2-B6 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029613 (Immunofluorescence)

by

Reveal Biosciences

No.

#029613

日期

2014.02.21

抗原

Lot Number

980993W

Method validated

Immunofluorescence

Positive Control

Mouse spleen

Negative Control

Mouse duodenum

Notes

Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.

Validation Images

Figure 1: AIF1 staining on mouse spleen (red), counterstained with DAPI (blue).

Figure 2: AIF1 staining on mouse duodenum (red), counterstained with DAPI (blue).

Figure 3: Isotype control staining on mouse spleen (red), counterstained with DAPI (blue).

Figure 4: Secondary only staining on mouse spleen (red), counterstained with DAPI (blue).
Full Methods
Primary Antibody

Antibody: AIF1
Catalog number: ABIN685477
Lot number: 980993W

Secondary Antibody

Antibody: Alexa Fluor 546 Donkey Anti Rabbit
Lot number: 1218269

Full Protocol

Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
Fixed slides were rinsed three times in PBS for 5 min each at RT.
Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
Sections were rinsed three times in PBS for 5 min each at RT.
Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.

Experimental Notes

None
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Ko, Shie, Lin, Chen, Yao, Chen: "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." in: Scientific reports, Vol. 7, Issue 1, pp. 17489, (2018) (PubMed).

Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, (2017) (PubMed).

Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, (2017) (PubMed).

Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, (2017) (PubMed).

Furukawa, Nagaike, Ozaki: "Databases for technical aspects of immunohistochemistry." in: Journal of toxicologic pathology, Vol. 30, Issue 1, pp. 79-107, (2017) (PubMed).

Kouam, Yuan, Njayou, He, Tsayem, Oladejo, Song, Moundipa, Gao: "Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity." in: Frontiers in pharmacology, Vol. 8, pp. 653, (2017) (PubMed).

Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, (2016) (PubMed).
抗原

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

别名

AIF1 (IBA1 产品)

别名

AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody

背景

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1
Background: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

基因ID

199

UniProt

P55008

途径

Smooth Muscle Cell Migration