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CSF1R 抗体 (pTyr723)

Name: CSF1R 抗体 (pTyr723)
SKU: ABIN683788
Availability: OutOfStock
Rating: 5 (1 reviews)

CSF1R 适用: 人, 犬 WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Colony Stimulating Factor 1 Receptor (CSF1R) (pTyr723) antibody (ABIN683788) (CSF1R 抗体 (pTyr723))

IHC

Staining of canine lymph node tissue with bs-3077r. (CSF1R 抗体 (pTyr723))

IHC (p)

2 images

(1 reference)

(1 validation)

产品编号 ABIN683788

发货至: 中国

Datasheet as PDF

抗原 See all CSF1R 抗体

CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))
抗原表位
22

21

21

19

16

16

14

11

10

8

7

7

6

6

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr723
适用
202

138

100

16

8

3

3

3

2

2

1

1

1
人, 犬
宿主
229

23

9

3

2
兔
Compatible Secondaries
克隆类型
231

34

1
多克隆
标记
95

26

15

13

13

9

6

6

6

6

6

6

5

5

5

5

5

5

5

5

5

4

4

1

1

1

1

1

1
This CSF1R antibody is un-conjugated
应用范围
169

101

82

65

49

25

23

20

11

5

5

4

4

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

犬, 人

预测反应

Mouse,Rat,Cow,Horse

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human CSF1R around the phosphorylation site of tyrosine 723

亚型

IgG

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anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 344-497) antibody
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anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 940-971) antibody
CSF1R 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Colony Stimulating Factor 1 Receptor (CSF1R) (Tyr809) antibody
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anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 21-120) antibody
CSF1R 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3G12 unconjugated

anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 20-152) antibody
CSF1R 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4C9G2 unconjugated

anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 895-923), (C-Term) antibody
CSF1R 适用: 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB34462 unconjugated

anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 895-923) antibody
CSF1R 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 201-300) antibody
CSF1R 适用: 人 ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 639-868) antibody
CSF1R 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #100091 (Immunohistochemistry)

by

Sveriges Lantbruksuniversitet

No.

#100091

日期

2016.11.24

抗原

CSF1R (Colony Stimulating Factor 1 Receptor)

Lot Number

9I02V3

Method validated

Immunohistochemistry

Positive Control

canine lymph node tissue

Negative Control

secondary antibody only

Notes

The CSF1R antibody ABIN683788 specifically labels the targeted antigen in canine lymph node samples in IHC.

Validation Images

Staining of canine lymph node tissue with ABIN683788. The primary antibody ABIN683788 was used at a 100 fold (A and B) and 500 fold (C and D) dilution. A negative control without primary antibody (E and F) was used to verify the specificity of the stain. Please refer to the protocol for detailed information. Images in the upper row (A, C, and E) were captured at 10x magnification, while the images below (B, D, and F) were taken at 20x magnification.
Full Methods
Primary Antibody

ABIN683788

Secondary Antibody

anti-rabbit (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712)

Full Protocol

Canine lymph node tissue is dissected and fixed in 4% buffered formalin ON at RT.
Deparaffinization and rehydration through graded xylene and graded alcohol series:
xylene twice for 3min.
100% ethanol for 1min.
95% ethanol for 1min.
70% ethanol for 1min.
50% ethanol for 1min.
H₂O for 2min.
Boil the sections in the decloaking chamber (Biocare Medical):
fill the outer chamber with 500ml distilled water.
fill a plastic jar with diluted Antigen unmasking solution (Vector, H3301) diluted 1:100.
Boil sections for 8 minutes, turn it off and leave for 1h.
Rinse sections with distilled water 3 times.
Rinse sections with 0.05M Tris-HCl pH7.6, 3 times.
Block sections in normal goat serum (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712) diluted 1:50 for 1h at RT.
Blot excess serum from sections; do not rinse.
Incubate sections with primary antibody rabbit-anti CSF1R antibody (antibodies-online, ABIN683788, lot 9I02V3) diluted 1:100-1:500 in with 0.05M Tris-HCl, pH7.6 ON at 4°C. Include no primary antibody negative controls.
Rinse sections with 0.05M Tris-HCl pH7.6, 3 times. Keep negative controls in a separate jar.
Incubate sections with biotinylated secondary antibody anti-rabbit (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712) diluted 1:200 for 30min at RT.
Mix 20µl of reagent A and reagent B of the Vectastain Elite ABC HRP kit (Vectorlabs, PK-6101, lot 300712) in 1ml 0.05M TrisHCl pH7.6 and leave the mixture for 30min at RT.
Rinse sections with 0.05M Tris-HCl pH7.6, 3 times.
Incubate sections in the ABC reagent mix for 45min at RT.
Rinse sections with 0.05M Tris-HCl pH7.6 3 times.
DAB staining using peroxidase substrate kit (Vector, product SK-4100, lot 020612):
Mix thoroughly 2.5ml and 1 drop buffer stock.
Add 2 drops DAB and mix thoroughly.
Add 1 drop H₂O₂ and mix thoroughly.
Rinse sections with 0.05M Tris-HCl pH7.6 3 times.
Stain sections with Mayer HTX (Histolab, 01820, lot 00811.) for 2-3min at RT.
Rinse sections in tap water for 5-10min.
Dehydrate in fresh ethanol:
95% ethanol twice for 2min.
99.5% ethanol twice for 2min.
xylene twice for 2min.
Mount sections in Pertex mounting medium (Histolab, 00811).
Dry sections in a ventilated place.
Image acquisition (x10 and x20 objectives).

Experimental Notes

The blocking step with avidin and biotin was omitted for the no primary antibody negative controls. As a result, there is some unspecific staining in these sections. ABIN683788 worked nicely on the canine lymph node that was used as a positive control.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Król, Majchrzak, Mucha, Homa, Bulkowska, Jakubowska, Karwicka, Paw?owski, Motyl: "CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells." in: BMC veterinary research, Vol. 9, pp. 65, (2013) (PubMed).
抗原

CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

别名

CSF1R (CSF1R 产品)

别名

AI323359 antibody, CD115 antibody, CSF-1R antibody, Csfmr antibody, Fim-2 antibody, Fms antibody, M-CSF-R antibody, M-CSFR antibody, C-FMS antibody, CSFR antibody, FIM2 antibody, FMS antibody, HDLS antibody, PDGFRB antibody, MGC89146 antibody, CSF-1-R antibody, c-fms antibody, mrfms antibody, CSF1 antibody, M-CSF antibody, csf1r antibody, fms antibody, panther antibody, colony stimulating factor 1 receptor antibody, macrophage colony-stimulating factor receptor antibody, colony stimulating factor 1 receptor, a antibody, Csf1r antibody, CSF1R antibody, m-csfr antibody, csf1r antibody, csf1ra antibody

背景

Synonyms: FMS, CSFR, FIM2, HDLS, C-FMS, CD115, CSF-1R, M-CSF-R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, Proto-oncogene c-Fms, CSF1R
Background: This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

基因ID

1436

UniProt

P07333

途径

RTK signaling, Inositol Metabolic Process, Cell-Cell Junction Organization