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Klotho 抗体 (AA 401-500)

Name: Klotho 抗体 (AA 401-500)
SKU: ABIN681778
Availability: OutOfStock

KL 适用: 人, 小鼠, 大鼠 IHC (p), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated

Mouse kidney lysates probed with Klotho Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (Klotho 抗体 (AA 401-500))

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Klotho 抗体 (AA 401-500))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-klotho Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (Klotho 抗体 (AA 401-500))

IHC (p)

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Klotho Polyclonal Antibody, Unconjugated (ABIN681778) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Klotho 抗体 (AA 401-500))

IHC

4 images

(1 reference)

产品编号 ABIN681778

发货至: 中国

Datasheet as PDF

抗原 See all Klotho (KL) 抗体

Klotho (KL)
抗原表位
4

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
AA 401-500
适用
31

28

12

7

6

6

5

5

4

3

3

2

1
人, 小鼠, 大鼠
宿主
41

4

2

1
兔
Compatible Secondaries
克隆类型
35

13
多克隆
标记
29

7

4

1

1

1

1

1

1

1

1
This Klotho antibody is un-conjugated
应用范围
35

22

12

9

7

4

4

3

2

1

1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Klotho

亚型

IgG

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anti-Klotho (KL) (AA 517-956) antibody
KL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Klotho (KL) (AA 720-769) antibody
KL 适用: 人, 大鼠, 兔, Cow, 犬, Pig, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Klotho (KL) (AA 251-300) antibody
KL 适用: 人, 小鼠, 大鼠, 兔, 马, 犬, 豚鼠, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Klotho (KL) (AA 986-1012), (C-Term) antibody
KL 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB21226 unconjugated

anti-Klotho (KL) (Middle Region) antibody
KL 适用: 人, 小鼠, 大鼠, 兔, 马, Cow, 犬, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Klotho (KL) (N-Term) antibody
KL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Klotho (KL) (Middle Region) antibody
KL 适用: 人, 小鼠, 大鼠, 兔, 马, Cow, 犬, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Klotho (KL) (Center) antibody
KL 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA, IF 宿主: 兔 Polyclonal unconjugated

anti-Klotho (KL) (AA 765-981) antibody
KL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Prudhomme, Glinka, Kurt, Liu, Wang: "The anti-aging protein Klotho is induced by GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells." in: Biochemical and biophysical research communications, Vol. 493, Issue 4, pp. 1542-1547, (2017) (PubMed).
抗原

Klotho (KL)

别名

Klotho (KL 产品)

别名

alpha-kl antibody, KL antibody, LOC100223456 antibody, klotho antibody, Klotho antibody, KL antibody, Kl antibody, kl antibody, LOC100533542 antibody

背景

Synonyms: Klotho, KL
Background: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

基因ID

9365

UniProt

Q9UEF7

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding