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MAPK14 抗体 (pThr180, pTyr182)

Name: MAPK14 抗体 (pThr180, pTyr182)
SKU: ABIN678668
Availability: OutOfStock
Rating: 5 (1 reviews)

MAPK14 适用: 人, 小鼠, 大鼠, 小鸡, 山羊 WB, ELISA, IHC (p), FACS, IF (cc) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN678668) (MAPK14 抗体 (pThr180, pTyr182))

IHC

Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (MAPK14 抗体 (pThr180, pTyr182))

Images provided by the Independent Validation Program (badge 29798). (MAPK14 抗体 (pThr180, pTyr182))

Images provided the Independent Validation Program (badge number 029662)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-phospho-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody at 1:250 overnight at room temperature followed by conjugation to secondary antibody. (MAPK14 抗体 (pThr180, pTyr182))

IHC (p)

7 images

(3 references)

(1 validation)

产品编号 ABIN678668

发货至: 中国

Datasheet as PDF

抗原 See all MAPK14 抗体

MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
抗原表位
39

32

18

16

16

12

12

10

8

7

6

6

6

6

6

5

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr180, pTyr182
适用
251

160

140

27

24

23

15

14

12

12

9

7

6

3

3

3

3

1

1

1

1

1
人, 小鼠, 大鼠, 小鸡, 山羊
宿主
221

31

2

1
兔
Compatible Secondaries
克隆类型
214

43
多克隆
标记
132

15

14

11

10

8

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

1

1
This MAPK14 antibody is un-conjugated
应用范围
198

111

75

48

42

42

37

28

28

21

10

10

5

5

4

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

交叉反应

小鸡, 山羊, 人, 小鼠, 大鼠

预测反应

Dog,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182)

亚型

IgG
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 299-360) antibody
MAPK14 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 8G4D11 unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 141-240) antibody
MAPK14 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), FACS, ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 19-279) antibody
MAPK14 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (Ser272) antibody
MAPK14 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 260-360) antibody
MAPK14 适用: 人 WB, ELISA, IP, IHC (p), PLA 宿主: 小鼠 Monoclonal 3D5 unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 299-360) antibody
MAPK14 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 10B11C8 unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 295-324), (C-Term) antibody
MAPK14 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB2723 unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) antibody
MAPK14 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (C-Term) antibody
MAPK14 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 299-360) antibody
MAPK14 适用: 人, 小鼠 WB, ELISA, IHC, IF/ICC, FACS 宿主: 小鼠 Monoclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029662 (Immunohistochemistry)

by

Immunohistochemistry Core, NYU Langone

No.

#029662

日期

2014.04.09

抗原

Lot Number

140106

Method validated

Immunohistochemistry

Positive Control

Human placenta tissue

Negative Control

Human tonsil tissue (no literature evidence of phospho-p38 MAPK in tonsil)

Notes

Signal is detected in positive control tissue and not in negative control tissue.

Validation Images

Figure 1: Human placenta tissue stained with anti-phospho-MAPK14 (brown) and counterstained with hematoxylin.

Figure 2: Human tonsil tissue stained with anti-phospho-MAPK14 (brown) and counterstained with hematoxylin.

Figure 3: Human placenta tissue stained with isotype control antibody (brown) and counterstained with hematoxylin.

Figure 4: Human placenta tissue stained with secondary antibody only (brown) and counterstained with hematoxylin.
Full Methods
Primary Antibody

Antigen: Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182)
Catalog number: ABIN678668
Lot number: 140106

Secondary Antibody

Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
Lot number: D07640BA

Full Protocol

Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
1. Slides were preheated in convection oven at 60°C for 30 min
2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
5. Slides rinsed with *reaction buffer* x3
6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
7. Slides rinsed with *reaction buffer*
8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
9. Slides rinsed with *reaction buffer* x3
10. *iView Biotinylated IgG* applied and incubated for 8 min
11. Slides rinsed with *reaction buffer*
14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
15. Slides rinsed with *reaction buffer*
16. *iView DAB/H2O2* applied and incubated for 8 min
17. Slides rinsed with *reaction buffer*
18. *iView Copper* applied and incubated for 4 min
19. Slides rinsed with *reaction buffer*
20. Slides washed in Dawn Detergent/tap water
21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software

Experimental Notes

Deviations from protocol/procedure supplied by manufacturer:
Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
Step 4: Italicized reagents and incubation time are fixed instrument parameters.
Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." in: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, (2016) (PubMed).

Li, Yu, Liu, Zhu, Hua: "Expression of miR-34c in response to overexpression of Boule and Stra8 in dairy goat male germ line stem cells (mGSCs)." in: Cell biochemistry and function, Vol. 31, Issue 4, pp. 281-8, (2013) (PubMed).

Wang, Sun, Liu, Yu, Li, Zeng, Chen, Jia: "Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1/2 signaling pathway: role in epithelial cell proliferation and migration." in: FEMS immunology and medical microbiology, Vol. 63, Issue 1, pp. 82-92, (2012) (PubMed).
抗原

MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

别名

p38 MAPK + (MAPK14 产品)

别名

CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, EXIP antibody, Mxi2 antibody, PRKM14 antibody, PRKM15 antibody, RK antibody, SAPK2A antibody, p38 antibody, p38ALPHA antibody, CRK1 antibody, Csbp1 antibody, Csbp2 antibody, Exip antibody, Hog antibody, Prkm14 antibody, Prkm15 antibody, Sapk2A antibody, p38Hog antibody, p38alpha antibody, p38b antibody, zp38b antibody, MAPK14 antibody, 186F5S antibody, BG:DS00797.3 antibody, CG7393 antibody, D-p38 antibody, D-p38 MAPK antibody, D-p38b antibody, Dm p38b antibody, Dmel\\CG7393 antibody, Dmp38b antibody, Dp38 antibody, Dp38b antibody, ESTS:186F5S antibody, Mpk34C antibody, anon-sts23 antibody, dp38b antibody, p38 MAPK antibody, p38 beta antibody, p38B antibody, p38Kb antibody, p38beta antibody, Crk1 antibody, p38-alpha antibody, p38MAPK antibody, p38a antibody, csbp antibody, mapk14a antibody, mxi2 antibody, sapk2 antibody, sapk2a antibody, AP22.98 antibody, AP22_98 antibody, ATMPK14 antibody, mitogen-activated protein kinase 14 antibody, SAPK2a antibody, MAP kinase 14A antibody, MAP kinase p38a antibody, MAPK 14A antibody, fk28c03 antibody, hm:zeh1243 antibody, wu:fk28c03 antibody, zp38a antibody, P38C-CRK antibody, mitogen-activated protein kinase 14 antibody, mitogen activated protein kinase 14 antibody, mitogen-activated protein kinase 14b antibody, p38b MAP kinase antibody, mitogen-activated protein kinase 14 S homeolog antibody, mitogen-activated protein kinase 14a antibody, CRK proto-oncogene, adaptor protein antibody, MAPK14 antibody, Mapk14 antibody, mapk14b antibody, p38b antibody, mapk14.S antibody, MPK14 antibody, mapk14a antibody, CRK antibody

背景

Synonyms: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, Stress-activated protein kinase 2a, MAPK14
Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

基因ID

1432

UniProt

Q16539

途径

MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins