LDL 抗体
(1 reference)-
- 抗原 See all LDL 抗体
- LDL (Low Density Lipoprotein (LDL))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LDL antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人
- 预测反应
- Mouse
- 纯化方法
- Purified by Protein A.
- 免疫原
- Full length plasma protein (Human)
- 亚型
- IgG
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- 应用备注
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ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "A novel assay uncovers an unexpected role for SR-BI in LDL transcytosis." in: Cardiovascular research, Vol. 108, Issue 2, pp. 268-77, (2015) (PubMed).
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: "A novel assay uncovers an unexpected role for SR-BI in LDL transcytosis." in: Cardiovascular research, Vol. 108, Issue 2, pp. 268-77, (2015) (PubMed).
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- 抗原
- LDL (Low Density Lipoprotein (LDL))
- 别名
- LDL (LDL 产品)
- 别名
- LDL cholesterol antibody, LDL antibody
- 背景
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Synonyms: _-Lipoprotein, Low density lipoprotein, apolipoprotein B100, apo-B100
Background: LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits, receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake. The protein component of LDL is apolipoprotein B100. LDL contains 2022 % protein, 1015 % triglycerides, 2028 % phospholipids, 3748 % cholesteryl esters and 810 % cholesterol.
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