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GAPDH 抗体 (AA 1-335)

Name: GAPDH 抗体 (AA 1-335)
SKU: ABIN678458
Availability: OutOfStock

GAPDH 适用: 人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody (ABIN678458) (GAPDH 抗体 (AA 1-335))

Paraformaldehyde-fixed, paraffin embedded Mouse brain Antigen retrieval by boiling in sodium citrate buffer (pH6. (GAPDH 抗体 (AA 1-335))

IHC (p)

Mouse muscle lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (GAPDH 抗体 (AA 1-335))

U251 lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (GAPDH 抗体 (AA 1-335))

Lane 1: Rat muscle lysates Lane 2: Rat heart lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (GAPDH 抗体 (AA 1-335))

Lane 1: Hela lysates Lane 2: MCF-7 lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (GAPDH 抗体 (AA 1-335))

Western blot analysis of SOX2, NANOG, and OCT protein. (GAPDH 抗体 (AA 1-335))

7 images

(16 references)

产品编号 ABIN678458

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
34

21

13

11

7

6

6

5

4

4

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-335
适用
212

144

137

69

63

51

35

27

22

20

19

19

17

15

11

11

11

9

9

8

4

4

4

4

4

3

3

2

2

2

2

2

1

1

1

1

1
人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species
宿主
159

94

17

11

4
兔
Compatible Secondaries
克隆类型
186

97
多克隆
标记
153

33

18

17

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
228

96

71

65

42

37

30

24

15

15

15

13

7

6

5

4

4

3

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鸡, Cow, 人, 小鼠, Pig, 兔, 大鼠, Various Species

纯化方法

Purified by Protein A.

免疫原

GAPDH protein of rabbit

亚型

IgG

Top Product

Discover our top product GAPDH Primary Antibody
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." in: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, (2018) (PubMed).

Sun, Zhang, Tang, Yang, Chu, Zhao: "SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer." in: International journal of nanomedicine, Vol. 12, pp. 2657-2672, (2017) (PubMed).

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Jia, Song, Yang, Ma, Li, Lu, Cao, Zhang, Zhu, Wang, Leng, Cao, Du, Xu: "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." in: Molecular medicine reports, Vol. 13, Issue 6, pp. 4627-35, (2016) (PubMed).

Wang, Jia, Chen, Wang, Li: "Osthole shows the potential to overcome P-glycoprotein‑mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway." in: Oncology reports, Vol. 35, Issue 6, pp. 3659-68, (2016) (PubMed).

Xiong, Luo, He, Xi, Lu, Li, Liu, Guo: "Up-regulation of miR-138 inhibits hypoxia-induced cardiomyocyte apoptosis via down-regulating lipocalin-2 expression." in: Experimental biology and medicine (Maywood, N.J.), Vol. 241, Issue 1, pp. 25-30, (2016) (PubMed).

Yang, Yao, Qian, Wei, Wu, Wang: "Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium." in: Cellular and molecular life sciences : CMLS, Vol. 72, Issue 24, pp. 4849-66, (2016) (PubMed).

Hou, Liu, Chai, Yu, Duan, Hu, Yin, Wang, Zhuang, Fan, Chu, Ma: "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." in: Molecular medicine reports, Vol. 12, Issue 2, pp. 2521-8, (2015) (PubMed).

Zhang, Shi, Lin, Ba, Cui: "MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy." in: Oncology reports, Vol. 34, Issue 3, pp. 1557-64, (2015) (PubMed).

Shao, Qian, Wang, Yan, Hu, Wang, Wang: "Construction of the plasmid coding for the expression of the EGFP-M-IL-2((88)Arg, (125)Ala) fusion protein and the anti-tumor effects exerted by the fusion protein in HeLa-60 cells." in: Oncology letters, Vol. 9, Issue 6, pp. 2729-2735, (2015) (PubMed).

Zhang, Feng, Tao, DE, Chang, Hu: "Increased stathmin expression strengthens fear conditioning in epileptic rats." in: Biomedical reports, Vol. 3, Issue 1, pp. 28-32, (2015) (PubMed).

Song, Xiao, Fu, Huang, Wang, Zhang, Yang: "Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 42, (2014) (PubMed).

Yan, Yang, Li, Duan, Wu, Qian, Li, Wang: "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." in: Journal of cardiovascular pharmacology, Vol. 64, Issue 4, pp. 310-7, (2014) (PubMed).

Steenblock, Heckel, Czupalla, Espírito Santo, Niehage, Sztacho, Hoflack: "The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts." in: The Journal of biological chemistry, Vol. 289, Issue 26, pp. 18347-59, (2014) (PubMed).

Li, McFadden, Morgan, Cui, Hu, Wan, Zeng: "All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts." in: Molecular vision, Vol. 16, pp. 689-97, (2010) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

Gapdh (GAPDH 产品)

别名

G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

背景

Synonyms: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, CDABP47, OK/SW-cl.12
Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

基因ID

2597

UniProt

P04406