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Ki-67 抗体 (AA 1201-1300)

MKI67 适用: 人, 小鼠, 大鼠, 兔, 山羊 ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. (Ki-67 抗体 (AA 1201-1300))

Image provided by One World Lab validation program. (Ki-67 抗体 (AA 1201-1300))

Formalin-fixed and paraffin-embedded human cervical carcinoma labeled with Rabbit Anti-Ki-67 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (Ki-67 抗体 (AA 1201-1300))

Paraformaldehyde-fixed, paraffin embedded rat testes tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-ki67 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining (Ki-67 抗体 (AA 1201-1300))

Formalin-fixed and paraffin-embedded human oral squamous cell carcinoma labeled with Rabbit Anti-Ki-67 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAPI staining (Ki-67 抗体 (AA 1201-1300))

Paraformaldehyde-fixed, paraffin embedded human stomach cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining. (Ki-67 抗体 (AA 1201-1300))

Paraformaldehyde-fixed, paraffin embedded human cervical cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining. (Ki-67 抗体 (AA 1201-1300))

7 images

(22 references)

产品编号 ABIN677858

发货至: 中国

Datasheet as PDF

抗原 See all Ki-67 (MKI67) 抗体

Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
抗原表位
16

16

16

16

15

12

7

7

7

6

4

4

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1201-1300
适用
180

66

40

5

2

1

1
人, 小鼠, 大鼠, 兔, 山羊
宿主
144

39

4

3
兔
Compatible Secondaries
克隆类型
135

53
多克隆
标记
83

13

13

7

7

5

5

5

5

5

5

5

5

5

5

5

2

2

2

2

2

2
This Ki-67 antibody is un-conjugated
应用范围
84

74

65

54

54

46

35

28

26

14

12

5

5

5

2

2

1

1

1
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Ki67

亚型

IgG
anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2293-2478) antibody
MKI67 适用: 人 IHC, WB, FACS, IF, StM 宿主: 小鼠 Monoclonal MKI67-2462 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2293-2478) antibody
MKI67 适用: 人 IHC, WB, FACS, IF, StM 宿主: 小鼠 Monoclonal MKI67-2461 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1160-1493) antibody
MKI67 适用: 人 IHC, WB, IHC (p) 宿主: 大鼠 Monoclonal 9C3 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2815-2928) antibody
MKI67 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1160-1493) antibody
MKI67 适用: 人 IHC, WB, ELISA, FACS 宿主: 小鼠 Monoclonal 7B12B1 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody
MKI67 适用: 人, Cow IHC, WB, FACS, ICC 宿主: 小鼠 Monoclonal Ki-67 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2924-3256) antibody
MKI67 适用: 人 IHC, IHC (p) 宿主: 小鼠 Monoclonal 8H5 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1160-1493) antibody
MKI67 适用: 人 IHC, IHC (p) 宿主: 大鼠 Monoclonal 5B10 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2860-3256) antibody
MKI67 适用: 人 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 3088-3235) antibody
MKI67 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Luo, Kong, Pei, Jin, Liu: "LncRNA CASC2 inhibits proliferation and migration of adenocarcinoma cells via miR-4735-3p and mTOR." in: Journal of cellular biochemistry, (2018) (PubMed).

Dong, Ma, Wang, Han, Zou, Wu, Zang, Zhuang: "YY1 promotes HDAC1 expression and decreases sensitivity of hepatocellular carcinoma cells to HDAC inhibitor." in: Oncotarget, Vol. 8, Issue 25, pp. 40583-40593, (2018) (PubMed).

Wu, Liu, Liu, Yan, Tang, Cui, Zhao, Shi, Hao, Yu, Qian: "A retrospective, single-center cohort study on 65 patients with primary retroperitoneal liposarcoma." in: Oncology letters, Vol. 15, Issue 2, pp. 1799-1810, (2018) (PubMed).

Thompson, Rahim, Arnold, Hielscher: "Loss of caveolin-1 alters extracellular matrix protein expression and ductal architecture in murine mammary glands." in: PLoS ONE, Vol. 12, Issue 2, pp. e0172067, (2017) (PubMed).

He, Chen, Yuan, Zhao, Chen, Wang, Li: "Tumor pH-Responsive Release of Drug-Conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy." in: ACS applied materials & interfaces, Vol. 9, Issue 38, pp. 32534-32544, (2017) (PubMed).

Liang, Fang, Yang, Song: "Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells." in: Acta histochemica, Vol. 120, Issue 1, pp. 56-63, (2017) (PubMed).

Antognelli, Gambelunghe, Muzi, Talesa et al.: "Glyoxalase I drives epithelial-to-mesenchymal transition via argpyrimidine-modified Hsp70, miR-21 and SMAD signalling in human bronchial cells BEAS-2B chronically exposed to crystalline silica ..." in: Free radical biology & medicine, Vol. 92, pp. 110-25, (2016) (PubMed).

Li, Zhu, Qiu: "Constructing aptamer anchored nanovesicles for enhanced tumor penetration and cellular uptake of water soluble chemotherapeutics." in: Acta biomaterialia, Vol. 35, pp. 269-79, (2016) (PubMed).

Drel, Tan, Barnes, Gorin, Lee, Wagner: "Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 9, pp. 3026-38, (2016) (PubMed).

Ma, Jia, Zhang: "IL-17 promotes keratinocyte proliferation via the downregulation of C/EBPα." in: Experimental and therapeutic medicine, Vol. 11, Issue 2, pp. 631-636, (2016) (PubMed).

Yang, Li, Zhu, Wang, Chen, Bai: "Hydroxysafflor yellow A inhibits angiogenesis of hepatocellular carcinoma via blocking ERK/MAPK and NF-?B signaling pathway in H22 tumor-bearing mice." in: European journal of pharmacology, Vol. 754, pp. 105-14, (2015) (PubMed).

Olianas, Dedoni, Onali et al.: "Antidepressants activate the lysophosphatidic acid receptor LPA(1) to induce insulin-like growth factor-I receptor transactivation, stimulation of ERK1/2 signaling and cell proliferation in CHO-K1 ..." in: Biochemical pharmacology, Vol. 95, Issue 4, pp. 311-23, (2015) (PubMed).

Zhang, Song, Pang, Zhang, Wan, Yuan, Wu, Liu: "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 14, pp. 4195-209, (2015) (PubMed).

Luo, Chen, Zhang, Chen, Li: "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 98, pp. 9-19, (2015) (PubMed).

Kim, Lim, Kim, Kim, Kim, Tian, Park, Park, Choung: "The oncoprotein, gankyrin, is up-regulated in middle ear cholesteatoma." in: Acta oto-laryngologica, Vol. 134, Issue 3, pp. 238-43, (2014) (PubMed).

Ma, Yu, He, Xu, Wu, Xia, Guo, Wang, Ye, Zhang, Tao: "Involvement of c-KIT mutation in the development of gastrointestinal stromal tumors through proliferation promotion and apoptosis inhibition." in: OncoTargets and therapy, Vol. 7, pp. 637-43, (2014) (PubMed).

Ye, Zheng, Fan, Qin, Li, Tang, Ai, Zhang, Bian, Dang, Huang, Zhou, Zhou, Xiong, Yan, Ma, Li: "Lactoferrin deficiency promotes colitis-associated colorectal dysplasia in mice." in: PLoS ONE, Vol. 9, Issue 7, pp. e103298, (2014) (PubMed).

Li, Li, Yi, Luo, Guo, Pan, Gu: "Amphiphilic peptide dendritic copolymer-doxorubicin nanoscale conjugate self-assembled to enzyme-responsive anti-cancer agent." in: Biomaterials, Vol. 35, Issue 35, pp. 9529-45, (2014) (PubMed).

Luo, Zhang, Chen, Wei, Zhang, Li: "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." in: International journal of pharmaceutics, Vol. 475, Issue 1-2, pp. 438-449, (2014) (PubMed).

Pan, She, Guo, Luo, Yi, Gu: "PEGylated dendritic diaminocyclohexyl-platinum (II) conjugates as pH-responsive drug delivery vehicles with enhanced tumor accumulation and antitumor efficacy." in: Biomaterials, Vol. 35, Issue 38, pp. 10080-92, (2014) (PubMed).

show more references
抗原

Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

别名

Ki-67 proliferation Marker (MKI67 产品)

别名

MKI67 antibody, kia antibody, mki67 antibody, MGC132156 antibody, D630048A14Rik antibody, Ki-67 antibody, Ki67 antibody, KIA antibody, MIB-1 antibody, marker of proliferation Ki-67 antibody, marker of proliferation Ki-67 L homeolog antibody, marker of proliferation Ki-67 S homeolog antibody, antigen identified by monoclonal antibody Ki 67 antibody, antigen identified by monoclonal antibody Ki-67 antibody, nucleolar protein interacting with the FHA domain of MKI67 antibody, MKI67 antibody, mki67 antibody, mki67.L antibody, mki67.S antibody, Mki67 antibody, NIFK antibody

背景

Synonyms: KIA, MIB-, MIB-1, PPP1R15, Antigen KI-67, MKI67
Background: Proliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

基因ID

4288

UniProt

P46013

途径

Glycosaminoglycan Metabolic Process

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