BACE1 抗体
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- 抗原 See all BACE1 抗体
- BACE1 (Beta-secretase 1 (BACE1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This BACE1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- 特异性
- Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.
- 纯化方法
- Protein G purified
- 免疫原
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Synthetic peptide from human BACE1.
Type of Immunogen: Synthetic peptide - 亚型
- IgG1
- Top Product
- Discover our top product BACE1 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, WB (1:10000)
Usage: Western Blot-This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute in 1 mL of Sterile PBS.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose.
- 注意事项
- Aviod frequent freeze thaws.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
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- 抗原
- BACE1 (Beta-secretase 1 (BACE1))
- 别名
- BACE1 / BACE (BACE1 产品)
- 别名
- ASP2 antibody, BACE antibody, HSPC104 antibody, C76936 antibody, Bace antibody, MGC145931 antibody, BACE1 antibody, zgc:77409 antibody, beta-secretase 1 antibody, beta-site APP cleaving enzyme 1 antibody, beta-site APP-cleaving enzyme 1 antibody, BACE1 antibody, Bace1 antibody, bace1 antibody
- 背景
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Name/Gene ID: BACE1
Subfamily: Aspartic A1
Family: Protease
Synonyms: BACE1, APP beta-secretase, Asp 2, ASP2, Aspartyl protease 2, Aspartylprotease 2, Beta-secretase, Beta-site APP-cleaving enzyme, BACE, HSPC104, KIAA1149, B-Secretase, Beta-secretase 1, Memapsin-2 - 基因ID
- 23621
- UniProt
- P56817
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