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BACE1 抗体

BACE1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN6774191
发货至: 中国
  • 抗原 See all BACE1 抗体
    BACE1 (Beta-secretase 1 (BACE1))
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This BACE1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Flow Cytometry (FACS)
    特异性
    Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.
    纯化方法
    Protein G purified
    免疫原
    Synthetic peptide from human BACE1.

    Type of Immunogen: Synthetic peptide
    亚型
    IgG1
    Top Product
    Discover our top product BACE1 Primary Antibody
  • 应用备注
    Approved: ELISA, Flo, WB (1:10000)

    Usage: Western Blot-This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in 1 mL of Sterile PBS.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS, 5 % trehalose.
    注意事项
    Aviod frequent freeze thaws.
    储存条件
    -20 °C
    储存方法
    Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
  • 抗原
    BACE1 (Beta-secretase 1 (BACE1))
    别名
    BACE1 / BACE (BACE1 产品)
    别名
    ASP2 antibody, BACE antibody, HSPC104 antibody, C76936 antibody, Bace antibody, MGC145931 antibody, BACE1 antibody, zgc:77409 antibody, beta-secretase 1 antibody, beta-site APP cleaving enzyme 1 antibody, beta-site APP-cleaving enzyme 1 antibody, BACE1 antibody, Bace1 antibody, bace1 antibody
    背景
    Name/Gene ID: BACE1
    Subfamily: Aspartic A1
    Family: Protease

    Synonyms: BACE1, APP beta-secretase, Asp 2, ASP2, Aspartyl protease 2, Aspartylprotease 2, Beta-secretase, Beta-site APP-cleaving enzyme, BACE, HSPC104, KIAA1149, B-Secretase, Beta-secretase 1, Memapsin-2
    基因ID
    23621
    UniProt
    P56817
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