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TNF alpha 抗体 (AA 181-235)

Name: TNF alpha 抗体 (AA 181-235)
SKU: ABIN677318
Availability: OutOfStock
Rating: 5 (1 reviews)

TNF alpha 适用: 人, 小鼠, 大鼠, 兔, 马 WB, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318) (TNF alpha 抗体 (AA 181-235))

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (TNF alpha 抗体 (AA 181-235))

Histology and immunofluorescence in rat corneal sections. (TNF alpha 抗体 (AA 181-235))

11 images

(21 references)

(1 validation)

产品编号 ABIN677318

发货至: 中国

Datasheet as PDF

Key Features
- High quality TNF alpha Primary Antibody for the detection of TNF alpha.
- Reliable product with high standard validation data
抗原 See all TNF alpha 抗体

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
抗原表位
43

32

15

14

14

13

10

6

6

5

5

5

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 181-235
适用
331

187

121

66

49

44

30

26

20

16

11

10

10

9

6

5

5

2

2

1

1

1

1
人, 小鼠, 大鼠, 兔, 马
宿主
271

192

28

25

6

2

2

1
兔
Compatible Secondaries
克隆类型
288

232
多克隆
标记
245

80

46

21

17

12

6

6

6

6

6

6

6

6

6

5

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This TNF alpha antibody is un-conjugated
应用范围
346

202

128

123

73

57

55

49

47

40

39

38

37

32

20

12

10

9

9

8

6

6

5

5

4

3

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

马, 人, 小鼠, 兔, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse TNF-alpha

亚型

IgG

Product Specific Information

What can the TNF alpha Antibody ABIN677318 be used for?

This polyclonal TNF alpha Antibody detects TNF alpha. The TNF alpha Antibody has been validated for various applications and can be used for the detection of TNF alpha and derivatives by Western Blotting, ELISA, Immunohistochemistry (Paraffin-embedded Sections), Immunofluorescence (Paraffin-embedded Sections), Immunohistochemistry (Frozen Sections).

What validation data is available for this TNF alpha Antibody?

The Primary Antibody is referenced in 20 publications and is characterized by a proven, very high reliability. It has currently 6 product images that show its performance in a variety of applications. TNF alpha Antibody for the detection of TNF alpha and derivatives.

What is the function of TNF alpha?

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008].
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibody
TNF alpha 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibody
TNF alpha 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibody
TNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)
TNF alpha 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MAb11 FITC

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibody
TNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200

限制

仅限研究用
生效 #029732 (Immunofluorescence)

by

Confocal Imaging Core, Beth Israel Deaconess Medical Center

No.

#029732

日期

2014.06.12

抗原

Lot Number

140113

Method validated

Immunofluorescence

Positive Control

Normal mouse lung tissue

Negative Control

Normal mouse adipose tissue

Notes

Fluorescent signal is detected in positive control, but not in negative control or isotype controls.

Validation Images

Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody

Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antibody

Panel 3: confocal image of positive control (mouse lung) with isotype control

Panel 4: confocal image of negative control (mouse adipose) with isotype control

Panel 5: confocal image of positive control (mouse lung) with secondary antibody only
Full Methods
Primary Antibody

Antibody: Tumor Necrosis Factor (TNF)
Catalog number: ABIN677318
Lot number: 140113

Secondary Antibody

Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
Lot number: Not available

Full Protocol

Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
Tissue sections were rinsed three times in TBS for 5 min each at RT.
Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
Tissue sections were rinsed three times in TBS for 5 min each at RT.
Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.

Experimental Notes

Nothing to note.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Maier, Reichhart, Gonnermann, Kociok, Riechardt, Gundlach, Strauß, Joussen: "Effects of TNFα receptor TNF-Rp55- or TNF-Rp75- deficiency on corneal neovascularization and lymphangiogenesis in the mouse." in: PLoS ONE, Vol. 16, Issue 4, pp. e0245143, (2021) (PubMed).

Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." in: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

Lennikov, Mirabelli, Mukwaya, Schaupper, Thangavelu, Lachota, Ali, Jensen, Lagali: "Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis." in: Angiogenesis, Vol. 21, Issue 2, pp. 267-285, (2019) (PubMed).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, (2018) (PubMed).

Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." in: Cytokine, Vol. 99, pp. 275-280, (2018) (PubMed).

Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, (2018) (PubMed).

Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." in: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, (2017) (PubMed).

Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, (2017) (PubMed).

Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." in: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, (2016) (PubMed).

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, (2016) (PubMed).

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." in: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, (2016) (PubMed).

Bertuglia, Pagliara, Grego, Ricci, Brkljaca-Bottegaro: "Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career." in: BMC veterinary research, Vol. 12, Issue 1, pp. 246, (2016) (PubMed).

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, (2015) (PubMed).

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, (2015) (PubMed).

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." in: BMC ophthalmology, Vol. 15, pp. 126, (2015) (PubMed).

Fang, Li, Sun, Bao, Ren, Lv, Ma: "Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption." in: International journal of molecular sciences, Vol. 14, Issue 5, pp. 10343-54, (2015) (PubMed).

Luo, Wang, Li, Wei, Zhang, Liu, Wang, Niu, Wang: "Maternal bisphenol a diet induces anxiety-like behavior in female juvenile with neuroimmune activation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 140, Issue 2, pp. 364-73, (2015) (PubMed).

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." in: PLoS ONE, Vol. 9, Issue 1, pp. e84387, (2014) (PubMed).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, (2014) (PubMed).
抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF alpha (TNF alpha 产品)

别名

DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody

背景

Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf
Background: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

基因ID

21926

UniProt

P06804

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome