Ret Proto-Oncogene 抗体 (AA 29-635)
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- 抗原 See all Ret Proto-Oncogene (RET) 抗体
- Ret Proto-Oncogene (RET)
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抗原表位
- AA 29-635
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Ret Proto-Oncogene antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- 特异性
- Recognizes human Ret. Species cross-reactivity: Mouse.
- 纯化方法
- Protein G purified
- 免疫原
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Recombinant protein corresponding to aa29-635 from human Ret/Fc Chimera expressed in Sf21 cells (P07949).
Type of Immunogen: Recombinant protein - 亚型
- IgG1
- Top Product
- Discover our top product RET Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 μg/mL, For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Western Blot: 1-2 μg/mL, Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhRet and rmRet is ~6 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with sterile PBS to a concentration of 0.5 mg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose.
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
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- 抗原
- Ret Proto-Oncogene (RET)
- 别名
- RET (RET 产品)
- 别名
- c-ret antibody, cret antibody, etID315074.13 antibody, ret1 antibody, wu:fd13h01 antibody, X-ret antibody, ret-A antibody, xret antibody, RET antibody, MTC1 antibody, CDHF12 antibody, CDHR16 antibody, HSCR1 antibody, MEN2A antibody, MEN2B antibody, PTC antibody, RET-ELE1 antibody, RET51 antibody, RET9 antibody, c-Ret antibody, ret proto-oncogene receptor tyrosine kinase antibody, ret proto-oncogene S homeolog antibody, ret proto-oncogene antibody, ret antibody, ret.S antibody, RET antibody, Ret antibody
- 背景
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Name/Gene ID: RET
Subfamily: Cadherin
Family: Protein Kinase
Synonyms: RET, Cadherin family member 12, CDHF12, C-ret, Hirschsprung disease 1, HSCR1, Hydroxyaryl-protein kinase, Oncogene ret, Proto-oncogene c-Ret, RET-ELE1, RET51, Ret proto-oncogene, RET transforming sequence, RET43, Men2-ret, MEN2B, MTC1, Receptor tyrosine kinase, CDHR16, MEN2A, PTC, RET9 - 基因ID
- 5979
- UniProt
- P07949
- 途径
- RTK signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation
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