MTOR 抗体 (AA 2436-2492)
(9 references)
(1 validation)-
- 抗原 See all MTOR (mTOR) 抗体
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
-
抗原表位
- AA 2436-2492
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This MTOR antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 小鸡, Cow, 鱼, 人, 小鼠, 大鼠
- 预测反应
- Dog,Cow,Sheep,Horse
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human mTOR
- 亚型
- IgG
-
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2388-2487) antibody
mTOR 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (C-Term) antibodymTOR 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibodymTOR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2400-2500) antibodymTOR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) antibodymTOR 适用: 人, 小鼠, 大鼠, 鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2311-2529) antibodymTOR 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 8H5A5 unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibodymTOR 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB13347 unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibodymTOR 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2448) antibodymTOR 适用: 人, 小鼠, 大鼠, Cow, 鱼 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2481) antibodymTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
IHC-P 1:100-500
IF(IHC-P) 1:50-200 - 限制
- 仅限研究用
-
- by
- Alamo Laboratories Inc
- No.
- #029761
- 日期
- 2014.07.03
- 抗原
- Lot Number
- 131209
- Method validated
- Western Blotting
- Positive Control
- HeLa cells
- Negative Control
- c6/36 mosquito cells (non-reactive species)
- Notes
- A strong band was observed in the positive control sample at the correct molecular weight. Multiple faint bands were also observed in the positive sample, but these bands did not represent a significant background signal. A band at the correct molecular weight was also observed in the negative control; however, it may be impossible to find a true negative control for this antigen.
- Primary Antibody
- Antigen: Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (1:200 dilution)
- Catalog number: ABIN676403
- Lot number: 131209
- Secondary Antibody
- Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
- Lot number: L170-6515
- Full Protocol
- Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
- 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
- The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
- Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
- The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
- The membrane was rinsed with TBST once.
- The membrane was immersed with the protein side up in the primary antibody solution (FRAP1; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
- The membrane was rinsed in TBST thrice for 5 min each.
- The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
- The membrane was rinsed thrice TBST thrice for 5 min each.
- The membrane was rinsed in TBS twice for 30 s each.
- Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
- The membrane was rinsed three times TBST.
- Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
- The membrane was washed in TBST 2 times for 10 min each.
- Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
- Experimental Notes
- - No experimental challenges noted.
生效 #029761 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-mTOR (upper panel) or with anti-Actin for loading control (lower panel).]()
Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-mTOR (upper panel) or with anti-Actin for loading control (lower panel).
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
-
-
: "Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis." in: Aging, Vol. 10, Issue 3, pp. 463-480, (2019) (PubMed).
: "Synergistic effect of copper and arsenic upon oxidative stress, inflammation and autophagy alterations in brain tissues of Gallus gallus." in: Journal of inorganic biochemistry, Vol. 178, pp. 54-62, (2018) (PubMed).
: "Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)." in: Journal of thermal biology, Vol. 70, Issue Pt B, pp. 37-45, (2017) (PubMed).
: "Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma." in: Oncology letters, Vol. 13, Issue 3, pp. 1437-1443, (2017) (PubMed).
: "Ranolazine improves oxidative stress and mitochondrial function in the atrium of acetylcholine-CaCl2 induced atrial fibrillation rats." in: Life sciences, Vol. 156, pp. 7-14, (2016) (PubMed).
: "Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo." in: Cancer prevention research (Philadelphia, Pa.), Vol. 9, Issue 1, pp. 53-62, (2016) (PubMed).
: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." in: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, (2015) (PubMed).
: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, (2014) (PubMed).
-
: "Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis." in: Aging, Vol. 10, Issue 3, pp. 463-480, (2019) (PubMed).
-
- 抗原
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- 别名
- Mtor/Frap (mTOR 产品)
- 别名
- FRAP1 antibody, FRAP antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody, 2610315D21Rik antibody, AI327068 antibody, Frap1 antibody, flat antibody, frap1 antibody, tor antibody, wu:fc22h08 antibody, mechanistic target of rapamycin kinase antibody, mechanistic target of rapamycin antibody, mechanistic target of rapamycin (serine/threonine kinase) antibody, MTOR antibody, Mtor antibody, mtor antibody
- 背景
-
Synonyms: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Background: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
- 基因ID
- 2475
- UniProt
- P42345
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
-
