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TJP1 抗体 (AA 1551-1702)

Name: TJP1 抗体 (AA 1551-1702)
SKU: ABIN675024
Availability: OutOfStock
Rating: 5 (1 reviews)

TJP1 适用: 人, 小鼠, 大鼠, Pig, Cow WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024) (TJP1 抗体 (AA 1551-1702))

IHC

Image kindly submitted by Kamlesh Gupta as part of the free sample program. (TJP1 抗体 (AA 1551-1702))

HQT regulates epithelial proliferation in the colonic mucosa of mice with DSS-induced acute and chronic colitis. (TJP1 抗体 (AA 1551-1702))

13 images

(7 references)

(1 validation)

产品编号 ABIN675024

发货至: 中国

Datasheet as PDF

抗原 See all TJP1 抗体

TJP1 (Tight Junction Protein 1 (TJP1))
抗原表位
15

6

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1551-1702
适用
65

43

36

19

17

9

6

5

4

4

2

2

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, Pig, Cow
宿主
61

3

2

1

1
兔
Compatible Secondaries
克隆类型
63

5
多克隆
标记
38

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This TJP1 antibody is un-conjugated
应用范围
51

19

18

14

13

12

11

11

11

7

7

3

2

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

Cow, 人, 小鼠, Pig, 大鼠

预测反应

Dog,Cow,Chicken,Rabbit,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human ZO-1

亚型

IgG
anti-Tight Junction Protein 1 (TJP1) (C-Term) antibody
TJP1 适用: 人, 小鼠, 大鼠, Pig, Cow, 犬, 豚鼠, 兔, 马, 猴, 小鸡, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (N-Term) antibody
KO Validated TJP1 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (Internal Region) antibody
Verified TJP1 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1600-1700) antibody
TJP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 适用: 人 WB, IHC, ELISA, FACS, FM 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1178-1527) antibody
TJP1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 适用: 小鼠 WB, IHC, IP 宿主: 大鼠 Monoclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1-190) antibody
TJP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 23-502) antibody
TJP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029577 (Immunohistochemistry)

by

Immunohistochemistry Core, NYU Langone

No.

#029577

日期

2014.01.18

抗原

Lot Number

130913

Method validated

Immunohistochemistry

Positive Control

Testis

Negative Control

See Controls section for negative controls

Notes

Signal was detected in positive control tissue, and not detected in a tissue expected to express very low levels of the target antigen.

Validation Images

Figure 1: TJP1 immunostaining of human testis (brown). Counterstain in blue.

Figure 2: Isotype control immunostaining of human testis (brown). Counterstain in blue.

Figure 3: Secondary only control immunostaining of human testis (brown). Counterstain in blue.

Figure 4: TJP1 immunostaining of human thymus (brown). Counterstain in blue.

Figure 4: Isotype control immunostaining of human thymus (brown). Counterstain in blue.

Figure 6: Secondary only immunostaining of human thymus (brown). Counterstain in blue.
Full Methods
Primary Antibody

Antibody: human Tight Junction Protein 1 (Zona Occludens 1) (TJP1)
Catalog number: ABIN675024
Lot number: 130913

Secondary Antibody

Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
Lot number: D05923BA

Full Protocol

Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
1. Slides were preheated in convection oven at 60°C for 30 minutes
2. Deparaffinization procedure: - 3 changes of Xylene, 5 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of 95% Ethanol, 3 minutes each - Rinsed in distilled water, 3 changes
1. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 minutes. - Slides were allowed to cool (in citrate) for 30 minutes. - Slides were washed x 3 in Distilled water
1. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
1. Slides rinsed with *reaction buffer* x 3
1. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 minutes
1. Slides rinsed with *reaction buffer*
1. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliters applied/slide) - Ventana Isotype control applied neat - Slides incubated overnight at room temperature (~12 hours ~25°C)
1. Slides rinsed with *reaction buffer* x3
1. *iVIEW Biotinylated IgG* applied and incubated for 8 minutes
1. Slides rinsed with *reaction buffer*
1. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 minutes
1. Slides rinsed with *reaction buffer*
1. *iVIEW DAB/H2O2* applied and incubated for 8 minutes
1. Slides rinsed with *reaction buffer*
1. *iVIEW Copper* applied and incubated for 4 minutes
1. Slides rinsed with *reaction buffer*
1. Slides washed in Dawn Detergent/tap water
1. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 minute - Decolorized (10% Acetic Acid in 70% ethanol), 1 minute - Slides washed in tap water, 1 minute - Bluing (Austin Clear Ammonia), 1 minute - Slides washed in tap water, 1 minute
1. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of Xylene, 5 minutes each - Mounted with Permount
1. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software

Experimental Notes

Deviations from protocol/procedure supplied by manufacturer (attached).
Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
Step 4: Italicized reagents and incubation time are fixed instrument parameters.
Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Tissue Interpretation (limited): - TJP1: Under the staining parameters described above, testis stained weakly (ducts) positive (Figure 1). Substantial signal detected in limited number of other tissues, including: breast, normal (NOS); pancreatic cancer (NOS), and stomach, normal (NOS). Most tissues showed low level of specific signal. Thymus did not have any detectable signal (Figure 4).
I-NC (Isotype negative control): No signal detected
B-NC (Blank negative control): No signal detected
Signal Localization: - Signal to noise was adequate with cytoplasmic, nuclear subcellular localization observed. Rare inner-membrane and no distinct membrane signal observed.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhuang, Zhong, Bian, Fan, Chen, Liu, Liu: "Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs." in: Life sciences, Vol. 216, pp. 168-175, (2019) (PubMed).

Kulhankova, Kinney, Stach, Gourronc, Grumbach, Klingelhutz, Salgado-Pabón: "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." in: Infection and immunity, (2018) (PubMed).

Zou, Lin, Li, Wu, He, Huang, Wang, Ye, Cheng, Ding, Zheng, Chi: "Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response." in: Scientific reports, Vol. 6, pp. 39299, (2018) (PubMed).

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, (2014) (PubMed).

Ruan, Liu, Zhou, Mi, Liu, Wu, Yao, Assaad, Deng, Hou, Wu, Yin: "Chlorogenic acid decreases intestinal permeability and increases expression of intestinal tight junction proteins in weaned rats challenged with LPS." in: PLoS ONE, Vol. 9, Issue 6, pp. e97815, (2014) (PubMed).

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, (2012) (PubMed).

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, (2011) (PubMed).
抗原

TJP1 (Tight Junction Protein 1 (TJP1))

别名

ZO-1 (TJP1 产品)

别名

ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody

背景

Synonyms: ZO-1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1
Background: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

基因ID

7082

UniProt

Q07157

途径

Carbohydrate Homeostasis, Cell-Cell Junction Organization