NFIC 抗体 (AA 323-372)
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- 抗原 See all NFIC 抗体
- NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))
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抗原表位
- AA 323-372
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适用
- 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, Pig, 猴, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NFIC antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NFIC
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (93%) Xenopus (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa323-372 of human NFIC (Q6FI30, NP_995315). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Pig, Guinea pig (100%), Zebra finch, Chicken (93%), Opossum (92%), Xenopus (86%), Stickleback (80%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product NFIC Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Aliquot to Avoid freeze/thaw cycles after reconstitution
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))
- 别名
- NFI / NFIC (NFIC 产品)
- 别名
- LOC100005568 antibody, 1110019L22Rik antibody, 1500041O16Rik antibody, AA589446 antibody, AI746521 antibody, NF1-C antibody, CTF antibody, CTF5 antibody, NF-I antibody, NFI antibody, CNFI-C antibody, nf1-c1 antibody, nuclear factor I/C antibody, nuclear factor I C antibody, nuclear factor I C L homeolog antibody, nfic antibody, NFIC antibody, Nfic antibody, nfic.L antibody
- 背景
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Name/Gene ID: NFIC
Synonyms: NFIC, CTF, CTF5, NF1-C, NF-I/C, NFI, NFI-C, TGGCA-binding protein, NF-I, Nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C - 基因ID
- 4782
- NCBI登录号
- NP_995315
- UniProt
- P08651
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