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EHD4 抗体 (AA 396-445)

EHD4 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠, 兔, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6744457
发货至: 中国
  • 抗原 See all EHD4 抗体
    EHD4 (EH-Domain Containing 4 (EHD4))
    抗原表位
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 396-445
    适用
    • 16
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠, 兔, Bat, 猴
    宿主
    • 14
    • 2
    克隆类型
    • 14
    • 2
    多克隆
    标记
    • 14
    • 1
    • 1
    This EHD4 antibody is un-conjugated
    应用范围
    • 16
    • 7
    • 4
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human EHD4
    预测反应
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide located between aa396-445 of human EHD4 (Q9H223, NP_644670). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Platypus, Lizard (100%), Elephant, Turkey, Zebra finch (92%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product EHD4 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    EHD4 (EH-Domain Containing 4 (EHD4))
    别名
    EHD4 (EHD4 产品)
    别名
    EHD4 antibody, past4 antibody, PAST4 antibody, 2210022F10Rik antibody, AI197390 antibody, AI846352 antibody, AV006278 antibody, Past2 antibody, EH domain containing 4 antibody, EH-domain containing 4 antibody, EH domain containing 4 L homeolog antibody, EHD4 antibody, ehd4 antibody, ehd4.L antibody, Ehd4 antibody
    背景
    Name/Gene ID: EHD4

    Synonyms: EHD4, EH domain-containing protein 4, EH-domain containing 4, EH domain containing 4, HCA10, HCA11, PAST4, Ortholog of rat pincher, PAST homolog 4
    基因ID
    30844
    NCBI登录号
    NP_644670
    UniProt
    Q9H223
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