LDHB 抗体 (C-Term)
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- 抗原 See all LDHB 抗体
- LDHB (Lactate Dehydrogenase B (LDHB))
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抗原表位
- C-Term
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适用
- 人, 兔, 犬, 猴, 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LDHB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human LDHB
- 预测反应
- Percent identity by BLAST analysis: Human, Dog, Rabbit, Chicken (100%) Mouse, Rat, Bovine, Horse, Pig (92%) Zebrafish (90%).
- 纯化方法
- Affinity purified
- 免疫原
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Synthetic peptide from C-Terminus of human LDHB (P07195, NP_002291). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Ferret, Dolphin, Elephant, Panda, Dog, Rabbit, Opossum, Turkey, Chicken (100%), Galago, Mouse, Rat, Hamster, Bovine, Horse, Pig, Guinea pig (92%), Catfish (91%), Zebrafish (90%), Zebra finch (85%), Salmon (84%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product LDHB Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- LDHB (Lactate Dehydrogenase B (LDHB))
- 别名
- LDHB / LDH-B (LDHB 产品)
- 别名
- LDH-B antibody, LDH-H antibody, LDHBD antibody, TRG-5 antibody, AI790582 antibody, H-Ldh antibody, Ldh-2 antibody, Ldh2 antibody, ldh-h antibody, ldh2 antibody, ldhb antibody, ldhba antibody, trg-5 antibody, LDHB antibody, LDH-B-A antibody, wu:fa16d07 antibody, wu:fa20c10 antibody, wu:fa96a08 antibody, LDH-B-B antibody, zgc:92882 antibody, lactate dehydrogenase B antibody, lactate dehydrogenase B S homeolog antibody, lactate dehydrogenase Ba antibody, lactate dehydrogenase Bb antibody, LDHB antibody, Ldhb antibody, ldhb.S antibody, ldhb antibody, ldhba antibody, ldhbb antibody
- 背景
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Name/Gene ID: LDHB
Synonyms: LDHB, LDH heart subunit, Lactate dehydrogenase H chain, Lactate dehydrogenase B, LDH-B, TRG-5, LDH-H, LDHBD - 基因ID
- 3945
- NCBI登录号
- NP_002291
- UniProt
- P07195
- 途径
- Warburg Effect
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