IGFBP4 抗体 (AA 180-229)
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- 抗原 See all IGFBP4 抗体
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
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抗原表位
- AA 180-229
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适用
- 人, 小鼠, 大鼠, Cow, 兔, 绵羊, 犬, 马, 山羊, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IGFBP4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human IGFBP4
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Sheep, Dog, Bovine, Horse (100%) Pig, Guinea pig, Chicken (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa180-229 of human IGFBP4 (P22692, NP_001543). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse (100%), Marmoset, Hamster, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (92%), Opossum (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product IGFBP4 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
- 别名
- IGFBP4 / IGFBP-4 (IGFBP4 产品)
- 别名
- IGFBP4 antibody, igfbp4 antibody, BP-4 antibody, HT29-IGFBP antibody, IBP4 antibody, IGFBP-4 antibody, AI875747 antibody, Deb2 antibody, IGF-BP4 antibody, IBP-4 antibody, insulin like growth factor binding protein 4 antibody, insulin-like growth factor-binding protein 4 antibody, insulin-like growth factor binding protein 4 antibody, IGFBP4 antibody, LOC100136305 antibody, LOC100194498 antibody, igfbp4 antibody, Igfbp4 antibody
- 背景
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Name/Gene ID: IGFBP4
Synonyms: IGFBP4, BP-4, HT29-IGFBP, IGF-binding protein 4, IBP-4, IGFBP-4, IBP4 - 基因ID
- 3487
- NCBI登录号
- NP_001543
- UniProt
- P22692
- 途径
- WNT signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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