ZNF777 抗体 (AA 468-517)
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- 抗原 See all ZNF777 抗体
- ZNF777 (Zinc Finger Protein 777 (ZNF777))
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抗原表位
- AA 468-517
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适用
- 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ZNF777 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ZNF777
- 预测反应
- Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa468-517 of human ZNF777 (Q9ULD5, NP_056509). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Gorilla, Marmoset, Dog, Bat (92%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product ZNF777 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- ZNF777 (Zinc Finger Protein 777 (ZNF777))
- 别名
- ZNF777 (ZNF777 产品)
- 别名
- 2500002G23Rik antibody, ZNF777 antibody, RGD1566056 antibody, Znf777 antibody, zinc finger protein 777 antibody, Zfp777 antibody, ZNF777 antibody, LOC769049 antibody
- 背景
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Name/Gene ID: ZNF777
Family: Zinc Finger
Synonyms: ZNF777, Zinc finger protein 777, KIAA1285 - 基因ID
- 27153
- NCBI登录号
- NP_056509
- UniProt
- Q9ULD5
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