PLEKHH2 抗体 (AA 1043-1092)
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- 抗原 See all PLEKHH2 products
- PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
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抗原表位
- AA 1043-1092
- 适用
- 人, 小鼠, 兔, Cow, 犬, 豚鼠, 马, Bat, 猴, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PLEKHH2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PLEKHH2
- 预测反应
- Percent identity by BLAST analysis: Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Rat (92%) Chicken (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa1043-1092 of human PLEKHH2 (Q8IVE3, NP_742066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%), Rat, Opossum (92%), Turkey, Chicken (86%), Xenopus, Drosophila (84%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
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- 应用备注
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Approved: IHC, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
- 别名
- PLEKHH2 (PLEKHH2 产品)
- 别名
- RGD1304935 antibody, si:ch73-105b23.3 antibody, PLEKHH1L antibody, AI256725 antibody, E030001K05 antibody, mKIAA2028 antibody, pleckstrin homology, MyTH4 and FERM domain containing H2 antibody, pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 antibody, Plekhh2 antibody, plekhh2 antibody, PLEKHH2 antibody
- 背景
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Name/Gene ID: PLEKHH2
Synonyms: PLEKHH2, KIAA2028, PLEKHH1L - 基因ID
- 130271
- NCBI登录号
- NP_742066
- UniProt
- Q8IVE3
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