ARID5A 抗体 (C-Term)
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- 抗原 See all ARID5A 抗体
- ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, 犬, 兔, 豚鼠, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ARID5A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ARID5A
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
- 别名
- MRF-1 / ARID5A (ARID5A 产品)
- 别名
- MRF-1 antibody, MRF1 antibody, RP11-363D14 antibody, D430024K22Rik antibody, Mrf1 antibody, AT-rich interaction domain 5A antibody, AT rich interactive domain 5A (MRF1-like) antibody, ARID5A antibody, Arid5a antibody, arid5a antibody
- 背景
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Name/Gene ID: ARID5A
Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I - 基因ID
- 10865
- NCBI登录号
- NP_997646
- UniProt
- Q03989
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