NR1I3 抗体 (AA 180-229)
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- 抗原 See all NR1I3 抗体
- NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
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抗原表位
- AA 180-229
- 适用
- 人, 小鼠, 大鼠, 犬, 豚鼠, Cow, Pig, 马, 兔, 猴, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NR1I3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NR1I3
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Bovine, Guinea pig (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa180-229 of human NR1I3 (Q6GZ85, NP_001070939). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Medaka (100%), Zebrafish (85%), Hamster, Turkey, Zebra finch, Chicken, Platypus, Lizard, Stickleback (84%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product NR1I3 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
- 别名
- NR1I3 / CAR (NR1I3 产品)
- 别名
- AA209988 antibody, AI551208 antibody, CAR antibody, CAR-beta antibody, Care2 antibody, ESTM32 antibody, MB67 antibody, CAR1 antibody, CXR antibody, nuclear receptor subfamily 1 group I member 3 antibody, nuclear receptor subfamily 1, group I, member 3 antibody, NR1I3 antibody, Nr1i3 antibody
- 背景
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Name/Gene ID: NR1I3
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: NR1I3, Car-beta, CAR1, Constitutive active receptor, Constitutive active response, Orphan nuclear receptor MB67, MB67, CAR - 基因ID
- 9970
- NCBI登录号
- NP_001070939
- UniProt
- Q14994
- 途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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