PODXL 抗体 (AA 301-350)
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- 抗原 See all PODXL 抗体
- PODXL (Podocalyxin-Like (PODXL))
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抗原表位
- AA 301-350
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PODXL antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PODXL
- 预测反应
- Percent identity by BLAST analysis: Human (100%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa301-350 of human PODXL (A6NHX8, NP_001018121). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey (100%), Gorilla (92%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product PODXL Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- PODXL (Podocalyxin-Like (PODXL))
- 别名
- PODXL / Podocalyxin (PODXL 产品)
- 别名
- Gp200 antibody, PC antibody, PCLP antibody, PCLP-1 antibody, PCB antibody, AW121214 antibody, Ly102 antibody, Pclp1 antibody, podocalyxin antibody, MEP21 antibody, PODXL antibody, Gp135 antibody, PCLP1 antibody, cPCLP1 antibody, podxl antibody, fc18d02 antibody, wu:fc18d02 antibody, Pc antibody, Pcb antibody, Podxl antibody, podocalyxin like antibody, pyruvate carboxylase antibody, podocalyxin-like antibody, PODXL antibody, PC antibody, Podxl antibody, podxl antibody, Pcx antibody
- 背景
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Name/Gene ID: PODXL
Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81 - 基因ID
- 5420
- NCBI登录号
- NP_001018121
- UniProt
- O00592
- 途径
- Tube Formation
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