ABCA5 抗体 (AA 1296-1345)
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- 抗原 See all ABCA5 抗体
- ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
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抗原表位
- AA 1296-1345
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适用
- 人, 犬, 兔, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ABCA5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ABCA5
- 预测反应
- Percent identity by BLAST analysis: Human (100%) Mouse, Rat (92%) Dog, Rabbit (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa1296-1345 of human ABCA5 (Q8WWZ7, NP_061142). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Mouse, Rat, Panda (92%), Bat (90%), Elephant, Dog, Rabbit, Horse, Opossum (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
- 别名
- ABCA5 (ABCA5 产品)
- 别名
- zgc:163009 antibody, ABC13 antibody, EST90625 antibody, B930033A02Rik antibody, mKIAA1888 antibody, ATP binding cassette subfamily A member 5 antibody, ATP-binding cassette, sub-family A (ABC1), member 5 antibody, ATP-binding cassette sub-family A member 5 antibody, ABCA5 antibody, abca5 antibody, LOC100545445 antibody, Abca5 antibody
- 背景
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Name/Gene ID: ABCA5
Subfamily: ATP-binding cassette - ABCA/ABC1
Family: Transporter
Synonyms: ABCA5, ATP-binding cassette A5, ABC13, EST90625, KIAA1888 - 基因ID
- 23461
- NCBI登录号
- NP_061142
- UniProt
- Q8WWZ7
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