PNPT1 抗体 (AA 489-538)
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- 抗原 See all PNPT1 抗体
- PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
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抗原表位
- AA 489-538
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适用
- 人, 大鼠, 犬, 马, Cow, 豚鼠, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PNPT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PNPT1
- 预测反应
- Percent identity by BLAST analysis: Human (100%) Mouse (92%) Rat, Guinea pig (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa489-538 of human PNPT1 (Q8TCS8, NP_149100). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse (92%), Rat, Guinea pig (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product PNPT1 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 50 μL sterile ddH2O.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
- 别名
- PNPT1 (PNPT1 产品)
- 别名
- COXPD13 antibody, DFNB70 antibody, OLD35 antibody, PNPASE antibody, old-35 antibody, 1200003F12Rik antibody, Old35 antibody, PNPase antibody, Pnptl1 antibody, polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody, polyribonucleotide nucleotidyltransferase 1 antibody, CpipJ_CPIJ005886 antibody, PNPT1 antibody, Pnpt1 antibody
- 背景
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Name/Gene ID: PNPT1
Synonyms: PNPT1, 3-5 RNA exonuclease OLD35, COXPD13, DFNB70, OLD35, PNPase 1, PNPase old-35, Old-35, PNPASE, Polynucleotide phosphorylase 1 - 基因ID
- 87178
- NCBI登录号
- NP_149100
- UniProt
- Q8TCS8
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