POLDIP3 抗体 (AA 326-375)
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- 抗原 See all POLDIP3 抗体
- POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))
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抗原表位
- AA 326-375
- 适用
- 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 猴, Bat, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLDIP3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human POLDIP3 / SKAR
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%) Xenopus (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa326-375 of human POLDIP3 (Q9BY77, NP_115687). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus (85%), Sea squirt (84%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product POLDIP3 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))
- 别名
- POLDIP3 / p46 (POLDIP3 产品)
- 别名
- PDIP46 antibody, SKAR antibody, 1110008P04Rik antibody, AA408269 antibody, AL022852 antibody, C77954 antibody, mKIAA1649 antibody, polymerase (DNA-directed), delta interacting protein 3 S homeolog antibody, DNA polymerase delta interacting protein 3 antibody, polymerase (DNA-directed), delta interacting protein 3 antibody, poldip3.S antibody, POLDIP3 antibody, Poldip3 antibody
- 背景
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Name/Gene ID: POLDIP3
Synonyms: POLDIP3, p46, KIAA1649, SKAR, RNA-binding protein P46, PDIP46, S6K1 Aly/REF-like target - 基因ID
- 84271
- NCBI登录号
- NP_115687
- UniProt
- Q9BY77
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