TRIM8 抗体 (AA 36-85)
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- 抗原 See all TRIM8 抗体
- TRIM8 (Tripartite Motif Containing 8 (TRIM8))
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抗原表位
- AA 36-85
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适用
- 人, 小鼠, 大鼠, 犬, Cow, 兔, Pig, 斑马鱼, Hamster, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TRIM8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human TRIM8 / GERP
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product TRIM8 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- TRIM8 (Tripartite Motif Containing 8 (TRIM8))
- 别名
- TRIM8 / GERP (TRIM8 产品)
- 别名
- GERP antibody, RNF27 antibody, AA408830 antibody, BB006755 antibody, Rnf27 antibody, si:ch211-208d15.5 antibody, wu:fc90g07 antibody, tripartite motif containing 8 antibody, tripartite motif containing 8 L homeolog antibody, tripartite motif-containing 8 antibody, tripartite motif containing 8b antibody, TRIM8 antibody, trim8.L antibody, Trim8 antibody, trim8b antibody
- 背景
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Name/Gene ID: TRIM8
Family: Tripartite Motif
Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8 - 基因ID
- 81603
- NCBI登录号
- NP_112174
- UniProt
- Q9BZR9
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