BACH2 抗体 (C-Term)
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- 抗原 See all BACH2 抗体
- BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))
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抗原表位
- C-Term
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适用
- 人, 兔, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This BACH2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human BACH2
- 预测反应
- Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Horse, Guinea pig (92%) Rabbit (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide from C-Terminus of human BACH2 (Q9BYV9, NP_068585). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Gibbon, Mouse, Rat, Horse, Guinea pig (92%), Galago, Elephant, Rabbit (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
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- 应用备注
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Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))
- 别名
- BACH2 (BACH2 产品)
- 别名
- E030004N02Rik antibody, Bach2 antibody, BTBD25 antibody, bach2 antibody, si:dkeyp-4f12.1 antibody, si:dkeyp-4f12.2 antibody, BTB and CNC homology, basic leucine zipper transcription factor 2 antibody, BTB domain and CNC homolog 2 antibody, BTB and CNC homology 1, basic leucine zipper transcription factor 2b antibody, Bach2 antibody, BACH2 antibody, bach2b antibody, bach2 antibody
- 背景
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Name/Gene ID: BACH2
Synonyms: BACH2, BTBD25, BTB and CNC homolog 2 - 基因ID
- 60468
- NCBI登录号
- NP_068585
- UniProt
- Q9BYV9
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