NKRF 抗体 (C-Term)
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- 抗原 See all NKRF 抗体
- NKRF (NFKB Repressing Factor (NKRF))
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抗原表位
- C-Term
- 适用
- 人, 小鼠, 大鼠, 犬, 马, 兔, Cow, 豚鼠, Pig, 猴, Bat, Hamster, 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NKRF antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NKRF
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide from C-Terminus of human NKRF (O15226, NP_060014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- NKRF (NFKB Repressing Factor (NKRF))
- 别名
- NKRF / NRF (NKRF 产品)
- 别名
- NKRF antibody, NRF antibody, nrf antibody, itba4 antibody, 9430034D17Rik antibody, AW124770 antibody, C80287 antibody, ITBA4 antibody, RGD1563950 antibody, NFKB repressing factor antibody, NFKB repressing factor L homeolog antibody, NF-kappaB repressing factor antibody, NFKB repressing factor-like antibody, NKRF antibody, nkrf antibody, nkrf.L antibody, Nkrf antibody, Nkrfl antibody
- 背景
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Name/Gene ID: NKRF
Synonyms: NKRF, NFkB-repressing factor, ITBA4, Protein ITBA4, NF-kappaB repressing factor, NFKB repressing factor, Transcription factor NRF, NF-kappa-B-repressing factor, NRF - 基因ID
- 55922
- NCBI登录号
- NP_060014
- UniProt
- O15226
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