ONECUT1 抗体 (C-Term)
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- 抗原 See all ONECUT1 抗体
- ONECUT1 (One Cut Homeobox 1 (ONECUT1))
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抗原表位
- C-Term
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适用
- 人, 大鼠, 犬, 兔, Pig, 马, 斑马鱼, Bat, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ONECUT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ONECUT1
- 预测反应
- Percent identity by BLAST analysis: Human, Rat, Dog, Rabbit, Pig (100%) Bovine (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide from C-Terminus of human ONECUT1 (Q9UBC0, NP_004489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Zebra finch (100%), Bovine (92%), Mouse, Opossum (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product ONECUT1 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- ONECUT1 (One Cut Homeobox 1 (ONECUT1))
- 别名
- ONECUT1 / HNF6 (ONECUT1 产品)
- 别名
- D9Ertd423e antibody, HNF-6 antibody, Hfh12 antibody, Hnf6 antibody, Hnf6b antibody, OC-1 antibody, Oc1 antibody, onecut antibody, onecut1 antibody, id:ibd5005 antibody, HNF6 antibody, HNF6A antibody, HNF-6a antibody, oc1 antibody, onecut1l antibody, zgc:65904 antibody, zgc:77450 antibody, one cut domain, family member 1 antibody, one cut homeobox 1 antibody, one cut domain, family member, like antibody, Onecut1 antibody, onecutl antibody, ONECUT1 antibody, onecut1 antibody
- 背景
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Name/Gene ID: ONECUT1
Synonyms: ONECUT1, Hepatocyte nuclear factor 6, HNF6, One cut domain family member 1, One cut homeobox 1, HNF-6, HNF6A - 基因ID
- 3175
- NCBI登录号
- NP_004489
- UniProt
- Q9UBC0
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