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SP4 抗体 (C-Term)

SP4 适用: 人, 大鼠, 小鼠, 马, 兔, Cow, 犬, 猴, Pig, 小鸡, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6741568
发货至: 中国
  • 抗原 See all SP4 抗体
    SP4 (Sp4 Transcription Factor (SP4))
    抗原表位
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    人, 大鼠, 小鼠, 马, 兔, Cow, 犬, 猴, Pig, 小鸡, 非洲爪蟾
    宿主
    • 8
    克隆类型
    • 8
    多克隆
    标记
    • 8
    This SP4 antibody is un-conjugated
    应用范围
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human SP4
    预测反应
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%) Guinea pig (92%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide from C-Terminus of human SP4 (Q02446, NP_003103). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Lizard, Xenopus (100%), Guinea pig (92%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    SP4 (Sp4 Transcription Factor (SP4))
    别名
    SP4 (SP4 产品)
    别名
    zgc:64226 antibody, DKFZp469M0517 antibody, HF1B antibody, SPR-1 antibody, HF-1b antibody, 5730497N03Rik antibody, HF1-b antibody, sp4 transcription factor antibody, Sp4 transcription factor antibody, Sp4 transcription factor L homeolog antibody, trans-acting transcription factor 4 antibody, sp4 antibody, SP4 antibody, sp4.L antibody, Sp4 antibody
    背景
    Name/Gene ID: SP4

    Synonyms: SP4, HF1B, Sp4 transcription factor, SPR-1, Transcription factor Sp4
    基因ID
    6671
    NCBI登录号
    NP_003103
    UniProt
    Q02446
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