SP4 抗体 (C-Term)
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- 抗原 See all SP4 抗体
- SP4 (Sp4 Transcription Factor (SP4))
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抗原表位
- C-Term
- 适用
- 人, 大鼠, 小鼠, 马, 兔, Cow, 犬, 猴, Pig, 小鸡, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SP4 antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human SP4
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%) Guinea pig (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide from C-Terminus of human SP4 (Q02446, NP_003103). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Lizard, Xenopus (100%), Guinea pig (92%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- SP4 (Sp4 Transcription Factor (SP4))
- 别名
- SP4 (SP4 产品)
- 别名
- zgc:64226 antibody, DKFZp469M0517 antibody, HF1B antibody, SPR-1 antibody, HF-1b antibody, 5730497N03Rik antibody, HF1-b antibody, sp4 transcription factor antibody, Sp4 transcription factor antibody, Sp4 transcription factor L homeolog antibody, trans-acting transcription factor 4 antibody, sp4 antibody, SP4 antibody, sp4.L antibody, Sp4 antibody
- 背景
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Name/Gene ID: SP4
Synonyms: SP4, HF1B, Sp4 transcription factor, SPR-1, Transcription factor Sp4 - 基因ID
- 6671
- NCBI登录号
- NP_003103
- UniProt
- Q02446
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