NRF1 抗体 (AA 360-409)
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- 抗原 See all NRF1 抗体
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
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抗原表位
- AA 360-409
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适用
- 人, 大鼠, Cow, 兔, 马, 豚鼠, 斑马鱼, 犬, 猴, 小鸡, Pig, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NRF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NRF1
- 无交叉反应
- 小鼠
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product NRF1 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
- 别名
- NRF1 / NRF-1 (NRF1 产品)
- 别名
- ALPHA-PAL antibody, C87038 antibody, D6Ertd415e antibody, SC:BZ3C13.1 antibody, SC:bZ3C13.2 antibody, ube2h antibody, Nfe2l1_retired antibody, IBR/F antibody, NRF1 antibody, alpha-pal antibody, nrf-1 antibody, LOC100220087 antibody, nuclear respiratory factor 1 antibody, nuclear respiratory factor 1 S homeolog antibody, NRF1 antibody, Nrf1 antibody, nrf1 antibody, nrf1.S antibody
- 背景
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Name/Gene ID: NRF1
Family: Transcription factor
Synonyms: NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL - 基因ID
- 4899
- NCBI登录号
- NP_005002
- UniProt
- Q16656
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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