TRIM35 抗体 (AA 359-408)
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- 抗原 See all TRIM35 抗体
- TRIM35 (Tripartite Motif Containing 35 (TRIM35))
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抗原表位
- AA 359-408
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适用
- 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, 犬, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TRIM35 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human TRIM35
- 预测反应
- Percent identity by BLAST analysis: Human, Dog, Bovine, Horse, Pig (100%) Rabbit (92%) Rat (91%) Mouse (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa359-408 of human TRIM35 (Q9UPQ4, NP_741983). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Marmoset, Bat (92%), Opossum (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product TRIM35 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- TRIM35 (Tripartite Motif Containing 35 (TRIM35))
- 别名
- TRIM35 (TRIM35 产品)
- 别名
- trim35 antibody, wu:fi37h05 antibody, zgc:63737 antibody, TRIM35 antibody, DKFZp468B2113 antibody, HLS5 antibody, MAIR antibody, 0710005M05Rik antibody, A430106H13Rik antibody, AW046487 antibody, Mair antibody, NC8 antibody, mKIAA1098 antibody, tripartite motif containing 35 antibody, tripartite motif containing 35-27 antibody, tripartite motif-containing 35 antibody, TRIM35 antibody, trim35-27 antibody, Trim35 antibody
- 背景
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Name/Gene ID: TRIM35
Family: Tripartite Motif
Synonyms: TRIM35, HLS5, KIAA1098, MAIR, Tripartite motif containing 35, Tripartite motif-containing 35 - 基因ID
- 23087
- NCBI登录号
- NP_741983
- UniProt
- Q9UPQ4
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