Sp3 抗体 (AA 287-336)
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- 抗原 See all Sp3 抗体
- Sp3 (Sp3 Transcription Factor (Sp3))
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抗原表位
- AA 287-336
- 适用
- 人, 小鼠, 大鼠, 豚鼠, 马, 兔, Cow, 犬, Bat, 猴, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Sp3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human SP3
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa287-336 of human SP3 (Q02447, NP_003102). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Chicken (92%), Turkey, Zebra finch, Platypus (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product Sp3 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- Sp3 (Sp3 Transcription Factor (Sp3))
- 别名
- SP3 (Sp3 产品)
- 别名
- SPR2 antibody, D130027J01Rik antibody, SP3 antibody, sp3 antibody, zgc:162922 antibody, Sp3 transcription factor antibody, trans-acting transcription factor 3 antibody, sp3a transcription factor antibody, Sp3 transcription factor S homeolog antibody, SP3 antibody, Sp3 antibody, sp3a antibody, sp3.S antibody
- 背景
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Name/Gene ID: SP3
Synonyms: SP3, SPR2, Transcription factor Sp3, Sp3 transcription factor, SPR-2, Specificity protein 3 - 基因ID
- 6670
- NCBI登录号
- NP_003102
- UniProt
- Q02447
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