PML 抗体 (AA 588-637)
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- 抗原 See all PML 抗体
- PML (Promyelocytic Leukemia (PML))
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抗原表位
- AA 588-637
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适用
- 人, 小鼠, 大鼠, Cow, 豚鼠, 马
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PML antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PML
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%) Pig (92%) Horse, Guinea pig (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa588-637 of human PML (P29590). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Bovine (100%), Marmoset, Panda, Bat, Rabbit, Pig (92%), Horse, Guinea pig (84%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product PML Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- PML (Promyelocytic Leukemia (PML))
- 别名
- PML (PML 产品)
- 别名
- MYL antibody, PP8675 antibody, RNF71 antibody, TRIM19 antibody, 1200009E24Rik antibody, AI661194 antibody, Trim19 antibody, RGD1562602 antibody, PML antibody, promyelocytic leukemia antibody, PML antibody, Pml antibody
- 背景
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Name/Gene ID: PML
Family: Transcription factor
Synonyms: PML, PP8675, Promyelocytic leukemia, Protein PML, TRIM19, Promyelocytic leukemia protein, RING finger protein 71, MYL, RNF71 - 基因ID
- 5371
- UniProt
- P29590
- 途径
- p53 Pathway, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
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