Ceramide Synthase 2 抗体 (AA 72-121)
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- 抗原 See all Ceramide Synthase 2 (CERS2) 抗体
- Ceramide Synthase 2 (CERS2)
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抗原表位
- AA 72-121
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适用
- 人, 豚鼠, Pig, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ceramide Synthase 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human LASS2
- 预测反应
- Percent identity by BLAST analysis: Pig (100%) Mouse, Horse (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa72-121 of human CERS2 (Q96G23, NP_071358). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Hamster, Pig, Guinea pig (100%), Orangutan, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Rabbit, Horse, Opossum (92%), Bovine, Bat (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product CERS2 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- Ceramide Synthase 2 (CERS2)
- 别名
- L3 / LASS2 (CERS2 产品)
- 别名
- L3 antibody, LASS2 antibody, SP260 antibody, TMSG1 antibody, 0610013I17Rik antibody, AI225939 antibody, Lass2 antibody, TRH3 antibody, ceramide synthase 2 antibody, CERS2 antibody, Cers2 antibody
- 背景
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Name/Gene ID: CERS2
Synonyms: CERS2, Ceramide synthase 2, LAG1 longevity assurance 2, L3, LASS2, SP260, TMSG1 - 基因ID
- 29956
- NCBI登录号
- NP_071358
- UniProt
- Q96G23
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